Protocol

Single Cell Protein Analysis

Volume 1346 of the series Methods in Molecular Biology pp 115-131

Multiplexed Peptide-MHC Tetramer Staining with Mass Cytometry

  • Mei Ling LeongAffiliated withSchool of Biological Sciences, Nanyang Technological University
  • , Evan W. NewellAffiliated withSingapore Immunology Network (SIgN), Agency for Science Technology and Research (A-STAR) Email author 

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Abstract

Mass cytometry is flow cytometry based on single cell mass spectrometry with decreased crosstalk between channels and an ability to probe >40 parameters per cell, making it well suited for multiplexed assays. Peptide major histocompatibility (MHC) tetramer staining allows direct detection of antigen specific cells and is also amenable to multiplexing/combinatorial approaches. Here we describe methods for multiplexed pMHC-tetramer staining using mass cytometry.

Key words

Mass cytometry Peptide-MHC tetramer Antigen-specific T cell Combinatorial tetramer staining