Abstract
Development of immunoblots is commonly performed using enzyme-labeled antibodies which convert soluble substrates into insoluble colored products. A simple, rapid, and sensitive alternative method which produces low background and allows a rapid quantitative evaluation is the use of radiolabeled antibodies or protein A conjugates. Here we describe the use of iodinated secondary antibodies for immunodetection of an autoantigen during HPLC purification.
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References
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Acknowledgement
Special thanks for excellent technical support to Joanne K. and Tim Gross (Oklahoma Medical Research Foundation, Oklahoma City, USA).
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Bartsch, H., Franke, C., Bachmann, M.P. (2015). Immunoblotting Using Radiolabeled Reagents for Detection. In: Kurien, B., Scofield, R. (eds) Detection of Blotted Proteins. Methods in Molecular Biology, vol 1314. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2718-0_9
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DOI: https://doi.org/10.1007/978-1-4939-2718-0_9
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-2717-3
Online ISBN: 978-1-4939-2718-0
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