Abstract
Sera of patients with systemic autoimmune diseases frequently contain autoantibodies to nuclear autoantigens. Immunoblotting of recombinant and native autoantigens is a commonly used technique for the identification and characterization of autoantibody specificities. Here we describe an easy procedure which facilitates the comparison of antibody specificities by reusing the same immunoblot for at least three times in order to detect an abundantly expressed autoantigen in total cellular extracts.
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Acknowledgement
Special thanks for excellent technical support to Joanne K. and Tim Gross (Oklahoma Medical Research Foundation, OK, USA).
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Bartsch, H., Bachmann, M.P. (2015). Sequential Use of Immunoblots for Characterization of Autoantibody Specificities. In: Kurien, B., Scofield, R. (eds) Detection of Blotted Proteins. Methods in Molecular Biology, vol 1314. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2718-0_18
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DOI: https://doi.org/10.1007/978-1-4939-2718-0_18
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-2717-3
Online ISBN: 978-1-4939-2718-0
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