Abstract
Western blotting has survived as an essential tool of the research and clinical laboratory because it has maintained its everyday scientific usefulness while simultaneously evolving as a facile weapon to confront high-content analytical demands of proteomic and diagnostic screening. New approaches for imaging blotted proteins have emerged to complement the core methodology.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
Köhler G, Milstein C (1975) Continuous cultures of fused cells secreting antibody of predefined specificity. Nature 256:495–497
Nowinski RC, Stone MR, Tam M, Lostrom ME, Burnette WN, O’Donnell PV (1980) Mapping of viral proteins with monoclonal antibodies: analysis of the envelope proteins of murine leukemia viruses. In: Kennett RH, McKearn TJ, Bechtol KB (eds) Monoclonal antibodies: hybridomas: a new dimension in biological analyses. Plenum, New York, pp 295–316
Burnette WN (1981) “Western Blotting”: electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A. Anal Biochem 112:195–203
Burnette WN (2009) Western blotting: remembrance of things past. In: Kurien BT, Scofield RH (eds) Protein blotting and detection: methods and protocols, vol 536. Humana, New York, pp 5–8
Burnette WN (2011) Western blotting. Clin Chem 57:132–133
Mukhopadhyay R (2012) W. Neal Burnette: the man behind the Western blot. ASBMB Today, January 2012, pp 17–19
Towbin H, Staehelin T, Gordon J (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A 76:4350–4354
Renart J, Reiser J, Stark G (1979) Transfer of proteins from gels to diazobenzyloxy-methyl-paper and detection with antisera: a method for studying antibody specificity and antigen structure. Proc Natl Acad Sci U S A 76:3116–3120
Mukhopadhyay R (2012) The men behind Western blotting. ASBMB Today, March 2012, pp 34–35
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2015 Springer Science+Business Media New York
About this protocol
Cite this protocol
Burnette, W.N. (2015). Western Blotting: Origin and Ascent of the Species. In: Kurien, B., Scofield, R. (eds) Detection of Blotted Proteins. Methods in Molecular Biology, vol 1314. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2718-0_1
Download citation
DOI: https://doi.org/10.1007/978-1-4939-2718-0_1
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-2717-3
Online ISBN: 978-1-4939-2718-0
eBook Packages: Springer Protocols