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Selective Disruption of the AKAP Signaling Complexes

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cAMP Signaling

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1294))

Abstract

Synthesis of the second messenger cAMP activates a variety of signaling pathways critical for all facets of intracellular regulation. Protein kinase A (PKA) is the major cAMP-responsive effector. Where and when this enzyme is activated has profound implications on the cellular role of PKA. A-Kinase Anchoring Proteins (AKAPs) play a critical role in this process by orchestrating spatial and temporal aspects of PKA action. A popular means of evaluating the impact of these anchored signaling events is to biochemically interfere with the PKA–AKAP interface. Hence, peptide disruptors of PKA anchoring are valuable tools in the investigation of local PKA action. This article outlines the development of PKA isoform-selective disruptor peptides, documents the optimization of cell-soluble peptide derivatives, and introduces alternative cell-based approaches that interrogate other aspects of the PKA–AKAP interface.

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Acknowledgements

This work was supported, in whole or in part, by National Institutes of Health Grants 1K22CA154600 to EJK, and DK105542 and DK054441 to JDS.

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Authors and Affiliations

  1. Department of Pharmaceutical and Biomedical Sciences, University of Georgia College of Pharmacy, Athens, GA, USA

    Eileen J. Kennedy

  2. Department of Pharmacology, Howard Hughes Medical Institute, University of Washington School of Medicine, 1959 NE Pacific Street, Box 357370, Seattle, WA, 98195, USA

    John D. Scott

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  1. Eileen J. Kennedy
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  2. John D. Scott
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Correspondence to John D. Scott .

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Editors and Affiliations

  1. Department of Physiology, Anatomy, and Genetics, University of Oxford, Oxford, United Kingdom

    Manuela Zaccolo

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Kennedy, E.J., Scott, J.D. (2015). Selective Disruption of the AKAP Signaling Complexes. In: Zaccolo, M. (eds) cAMP Signaling. Methods in Molecular Biology, vol 1294. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2537-7_11

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  • DOI: https://doi.org/10.1007/978-1-4939-2537-7_11

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