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Isolation of Adult Stem Cell Populations from the Human Cornea

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Stem Cell Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1235))

Abstract

Corneal blindness is a leading cause of vision loss globally. From a tissue engineering perspective, the cornea represents specific challenges in respect to isolating, stably expanding, banking, and effectively manipulating the various cell types required for effective corneal regeneration. The current research trend in this area focuses on a combined stem cell component with a biological or synthetic carrier or engineering scaffold. Corneal derived stem cells play an important role in such strategies as they represent an available supply of cells with specific abilities to further generate corneal cells in the long term. This chapter describes the isolation protocols of the epithelial stromal and endothelial stem cell populations.

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Acknowledgements

Matthew Branch is funded by Fight for Sight. Corneal tissue for this work is kindly supplied by Manchester Eye Bank and Rakesh Jayaswal at the Eye Unit, Queen Alexandra Hospital, Portsmouth. The authors also wish to thank Laura Sidney for providing her help in reviewing the MACS sections. Wing-Yan Yu was funded by the Postgraduate Scholarship awarded from the University of Hong Kong.

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Authors and Affiliations

  1. Ophthalmology DCN, University of Nottingham, Queen’s Medical Center, Clifton Blvd., Nottingham, NG7 2UH, UK

    Matthew J. Branch, Wing-Yan Yu & Andrew Hopkinson

  2. Institute of Ageing and Chronic Disease, University of Liverpool, Liverpool, UK

    Carl Sheridan

Authors
  1. Matthew J. Branch
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  2. Wing-Yan Yu
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  3. Carl Sheridan
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  4. Andrew Hopkinson
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Corresponding author

Correspondence to Andrew Hopkinson .

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Editors and Affiliations

  1. HemoGenix, Inc., Colorado Springs, Colorado, USA

    Ivan N. Rich

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Branch, M.J., Yu, WY., Sheridan, C., Hopkinson, A. (2015). Isolation of Adult Stem Cell Populations from the Human Cornea. In: Rich, I. (eds) Stem Cell Protocols. Methods in Molecular Biology, vol 1235. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1785-3_14

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  • DOI: https://doi.org/10.1007/978-1-4939-1785-3_14

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1784-6

  • Online ISBN: 978-1-4939-1785-3

  • eBook Packages: Springer Protocols

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