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Potato (Solanum tuberosum L.)

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Agrobacterium Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1224))

Abstract

Agrobacterium-mediated transformation is the most common method for the incorporation of foreign genes into the genome of potato as well as many other species in the Solanaceae family. This chapter describes protocols for the genetic transformation of three species of potato: Solanum tuberosum subsp. tuberosum (Desiréé), S. tuberosum subsp. andigenum (Blue potato), and S. tuberosum subsp. andigena using internodal segments as explants.

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References

  1. Potato (2013) Retrieved from http://cipotato.org/potato

  2. Facts and figures about potato (2013) Retrieved from http://cipotato.org/potato/publications/pdf/005449.pdf

  3. Xu X, Pan S, Cheng S, Zhang B, Mu D, Ni P, Visser RG (2011) Genome sequence and analysis of the tuber crop potato. Nature 475:189–195

    Article  CAS  PubMed  Google Scholar 

  4. Bradeen JM, Carputo D, Douches D (2009) Part 7 Transgenic sugar, tuber and fiber crops. doi:10.1002/9781405181099. k0704 in compendium of transgenic crop plants

  5. De Block M (1998) Genotype-independent leaf disc transformation of potato (Solanum tuberosum) using Agrobacterium tumefaciens. Theor Appl Genet 76:767–774

    Article  Google Scholar 

  6. Stiekema WJ, Heidekamp F, Louwerse JD, Verhoeven HA, Dijkhuis P (1998) Introduction of foreign genes into potato cultivars Bintje and Desiree using an Agrobacterium tumefaciens binary vector. Plant Cell Rep 7:47–50

    Article  Google Scholar 

  7. Sheerman S, Bevan MW (1988) A rapid transformation method for Solanum tuberosum using binary Agrobacterium tumefaciens vectors. Plant Cell Rep 7:13–15

    Article  CAS  PubMed  Google Scholar 

  8. Tavazza R, Tavazza M, Ordas RJ, Ancora G, Benvenuto E (1988) Genetic transformation of potato (Solanum tuberosum): an efficient method to obtain transgenic plants. Plant Sci 59:175–181

    Article  CAS  Google Scholar 

  9. Wenzler H, Mignery G, May G, Park A (1989) A rapid and efficient transformation method for the production of large numbers of transgenic potato plants. Plant Sci 63:79–85

    Article  CAS  Google Scholar 

  10. Mitten DH, Horn M, Burrel MM, Blundy KS (1990) Strategies for potato transformation and regeneration. In: Vayda ME, Park WD (eds) The molecular and cellular biology of the potato. CAB International, Wallingford, pp 181–191

    Google Scholar 

  11. Beaujean A, Sangwan RS, Lecardonnel A, Sangwan-Norreel BS (1998) Agrobacterium-mediated transformation of three economically important potato cultivars using sliced internodal explants: an efficient protocol of transformation. J Exp Bot 49:1589–1595

    Google Scholar 

  12. Millam S (2006) Potato (Solanum tuberosum L.). In: Wang K (ed) Agrobacterium protocols, vol 2, 2nd edn. Humana, Totowa, NJ, pp 25–35

    Chapter  Google Scholar 

  13. Chakravarty B, Wang-Pruski G (2010) Rapid regeneration of stable transformants in cultures of potato by improving factors influencing Agrobacterium-mediated transformation. Adv Biosci Biotechnol 1:409–416

    Article  CAS  Google Scholar 

  14. Trujillo C, Rodriguez-Arango E, Jaramillo S, Hoyos R, Orduz S, Arango R (2001) One step transformation of two Andean potato cultivars (Solanum tuberosum L. subsp. andigena). Plant Cell Rep 20:637–641

    CAS  Google Scholar 

  15. Banerjee AK, Prat S, Hannapel DJ (2006) Efficient production of transgenic potato (S. tuberosum L. ssp. andigena) plants via Agrobacterium tumefaciens-mediated transformation. Plant Sci 170:732–738

    Article  CAS  Google Scholar 

  16. Narváez-Vásquez J, Ryan AC (2002) The systemin precursor gene regulates both defensive and developmental genes in Solanum tuberosum. Proc Natl Acad Sci U S A 99:15818–15821

    Article  PubMed Central  PubMed  Google Scholar 

  17. Jefferson RA, Kavanagh TA, Bevan MW (1987) GUS fusion: glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J 6:3901–3907

    CAS  PubMed Central  PubMed  Google Scholar 

  18. Po-Yen C, Chen-Kuen W, Shaw-Ching S, Kin-Ying T (2003) Complete sequence of the binary vector pBI121 and its application in cloning T-DNA insertion from transgenic plants. Mol Breeding 11:287–293

    Article  Google Scholar 

  19. Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res 50: 151–158

    Google Scholar 

  20. Murashige T, Skoog F (1962) A revised medium for rapid growth and bio-assays with tobacco tissue cultures. Physiol Plant 15: 473–497

    Google Scholar 

  21. Sambrook J, Russell DW (2001) Molecular cloning: a laboratory manual (3rd ed), CSHL Press, New York, NY 3:1–1453

    Google Scholar 

  22. Hoagland DR, Arnon DI (1950) The water culture method for growing plants without soil. University of California. Agric. Exp. Station, Berkeley

    Google Scholar 

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Authors and Affiliations

  1. Plant Transformation Research Center, University of California Riverside, South Campus Dr. Head House No.2, Riverside, CA, 92507-0124, USA

    Venkateswari J. Chetty, Javier Narváez-Vásquez & Martha L. Orozco-Cárdenas Ph.D.

Authors
  1. Venkateswari J. Chetty
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  2. Javier Narváez-Vásquez
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  3. Martha L. Orozco-Cárdenas Ph.D.
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Corresponding author

Correspondence to Martha L. Orozco-Cárdenas Ph.D. .

Editor information

Editors and Affiliations

  1. Plant Sciences Institute, and Department of Agronomy, Center for Plant Transformation, Iowa State University, Ames, Iowa, USA

    Kan Wang

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Chetty, V.J., Narváez-Vásquez, J., Orozco-Cárdenas, M.L. (2015). Potato (Solanum tuberosum L.). In: Wang, K. (eds) Agrobacterium Protocols. Methods in Molecular Biology, vol 1224. Springer, New York, NY. https://doi.org/10.1007/978-1-4939-1658-0_8

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  • DOI: https://doi.org/10.1007/978-1-4939-1658-0_8

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  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4939-1657-3

  • Online ISBN: 978-1-4939-1658-0

  • eBook Packages: Springer Protocols

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