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Cell Cycle Measurement of Mouse Hematopoietic Stem/Progenitor Cells

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Hematopoietic Stem Cell Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1185))

Abstract

Lifelong production of blood cells is sustained by hematopoietic stem cells (HSC). HSC reside in a mitotically quiescent state within specialized areas of the bone marrow (BM) microenvironment known as the hematopoietic niche (HN). HSC enter into active phases of cell cycle in response to intrinsic and extrinsic biological cues thereby undergoing differentiation or self-renewal divisions. Quiescent and mitotically active HSC have different metabolic states and different functional abilities such as engraftment and BM repopulating potential following their transplantation into conditioned recipients. Recent studies reveal that various cancers also utilize the same mechanisms of quiescence as normal stem cells and preserve the root of malignancy thus contributing to relapse and metastasis. Therefore, exploring the stem cell behavior and function in conjunction with their cell cycle status has significant clinical implications in HSC transplantation and in treating cancers. In this chapter, we describe methodologies to isolate or analytically measure the frequencies of quiescent (G0) and active (G1, S, and G2–M) hematopoietic progenitor and stem cells among murine BM cells.

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Acknowledgements

The authors thank the operators of the Indiana University Melvin and Bren Simon Cancer Center Flow Cytometry Resource Facility for their outstanding technical help and support. Indiana University is an NIDDK designated Center of Excellence in Molecular Hematology (NIDDK P01 DK090948). The Flow Cytometry Research Facility is partially funded by NCI P30CA082709.

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Authors and Affiliations

  1. Indiana University School of Medicine, 980 W. Walnut Street, R3-312, Indianapolis, IN, 46202, USA

    Brahmananda Reddy Chitteti & Edward F. Srour

Authors
  1. Brahmananda Reddy Chitteti
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  2. Edward F. Srour
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Corresponding author

Correspondence to Brahmananda Reddy Chitteti .

Editor information

Editors and Affiliations

  1. Department of Pediatrics, Aflac Cancer and Blood Disorders Center Emory University School of Medicine, Atlanta, Georgia, USA

    Kevin D. Bunting

  2. Department of Pediatrics, Emory University School of Medicine Aflac Cancer & Blood Disorders Ctr, Atlanta, Georgia, USA

    Cheng-Kui Qu

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Chitteti, B.R., Srour, E.F. (2014). Cell Cycle Measurement of Mouse Hematopoietic Stem/Progenitor Cells. In: Bunting, K., Qu, CK. (eds) Hematopoietic Stem Cell Protocols. Methods in Molecular Biology, vol 1185. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1133-2_5

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  • DOI: https://doi.org/10.1007/978-1-4939-1133-2_5

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1132-5

  • Online ISBN: 978-1-4939-1133-2

  • eBook Packages: Springer Protocols

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