Abstract
Instructions for proper alignment for each of the most common wide field light microscopy contrast techniques are presented: bright field, polarization, dark field, phase contrast, differential interference contrast, and fluorescence. These are then followed by methods on photomicrography, measurement, and cleaning. An extensive set of Notes provides practical tips for each of these methods.
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Acknowledgements
The author wishes to thank Olympus America INC. for the loan of a BX43 microscope used in many of the figures. He also wishes to thank Carl Zeiss Microscopy LLC for providing several of the illustrations.
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© 2014 Springer Science+Business Media, New York
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Bagnell, R. (2014). Light Microscope Alignment Methods. In: Day, C. (eds) Histopathology. Methods in Molecular Biology, vol 1180. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1050-2_4
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DOI: https://doi.org/10.1007/978-1-4939-1050-2_4
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-1049-6
Online ISBN: 978-1-4939-1050-2
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