Abstract
Identification of eosinophils in whole blood samples by flow cytometry is often problematic. There are usually only a low number and percentage of cells that may be detected, and it may be difficult to discriminate eosinophils from other granulocytes. Here, we propose a simple approach using the eosinophil’s intrinsic autofluorescence properties, combined with detection of CCR3 expression, to reliably identify eosinophils in a mixed leukocyte population.
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Acknowledgements
This work was supported by funding from Agriculture Life and Environmental Science (ALES) and Campus Saint-Jean research funds from University of Alberta to FD.
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Ethier, C., Lacy, P., Davoine, F. (2014). Identification of Human Eosinophils in Whole Blood by Flow Cytometry. In: Walsh, G. (eds) Eosinophils. Methods in Molecular Biology, vol 1178. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1016-8_8
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DOI: https://doi.org/10.1007/978-1-4939-1016-8_8
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-1015-1
Online ISBN: 978-1-4939-1016-8
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