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Engineering and Analyzing Multicellular Systems

Volume 1151 of the series Methods in Molecular Biology pp 39-47

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Combining Engineering and Evolution to Create Novel Metabolic Mutualisms Between Species

  • Lon ChubizAffiliated withDepartment of Organismic and Evolutionary Biology, Harvard University
  • , Sarah DouglasAffiliated withDepartment of Organismic and Evolutionary Biology, Harvard University
  • , William HarcombeAffiliated withDepartment of Ecology, Evolution, and Behavior, University of Minnesota Email author 

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Abstract

Synthetic communities can be used as model systems for the molecular examination of species interactions. Manipulating synthetic communities to create novel beneficial interactions provides insight into the mechanisms of cooperation as well as the potential to improve the productivity of industrially relevant systems. Here, we present a general scheme for evolving a mutualism from a bacterial consortium in which one species consumes the by-products of another.

Key words

Synthetic ecology Consortia Cooperation Cross-feeding Evolution