Abstract
Transgenesis of rearranged α and β chains from the T-cell receptor has allowed the generation of a variety of mice with a predetermined T-cell repertoire. These mice have been extensively used as tools to circumvent the low precursor frequency of naturally occurring endogenous T cells. As such, they have been valuable to study pathways of T-cell development in the thymus. In addition, these mice can also be considered as a valuable source of naive and/or memory T cells with a defined specificity. I will comment in this chapter the use of this source of T cells with known antigen reactivity to study in vivo T-cell behavior in the periphery, including during autoimmune responses.
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References
Ochsenbein AF, Klenerman P, Karrer U, Ludewig B, Pericin M, Hengartner H et al (1999) Immune surveillance against a solid tumor fails because of immunological ignorance. Proc Natl Acad Sci U S A 96(5):2233–2238
Lyons AB, Parish CR (1994) Determination of lymphocyte division by flow cytometry. J Immunol Methods 171(1):131–137
Kurts C, Sutherland RM, Davey G, Li M, Lew AM, Blanas E et al (1999) CD8 T cell ignorance or tolerance to islet antigens depends on antigen dose. Proc Natl Acad Sci U S A 96(22):12703–12707
Hernandez J, Aung S, Marquardt K, Sherman LA (2002) Uncoupling of proliferative potential and gain of effector function by CD8(+) T cells responding to self-antigens. J Exp Med 196(3):323–333
Kurts C, Carbone FR, Barnden M, Blanas E, Allison J, Heath WR et al (1997) CD4+ T cell help impairs CD8+ T cell deletion induced by cross-presentation of self-antigens and favors autoimmunity. J Exp Med 186(12):2057–2062
Schulz O, Diebold SS, Chen M, Naslund TI, Nolte MA, Alexopoulou L et al (2005) Toll-like receptor 3 promotes cross-priming to virus-infected cells. Nature 433(7028):887–892
Kurts C, Heath WR, Kosaka H, Miller JF, Carbone FR (1998) The peripheral deletion of autoreactive CD8+ T cells induced by cross-presentation of self-antigens involves signaling through CD95 (Fas, Apo-1). J Exp Med 188(2):415–420
Hernandez J, Aung S, Redmond WL, Sherman LA (2001) Phenotypic and functional analysis of CD8(+) T cells undergoing peripheral deletion in response to cross-presentation of self-antigen. J Exp Med 194(6):707–717
Redmond WL, Marincek BC, Sherman LA (2005) Distinct requirements for deletion versus anergy during CD8 T cell peripheral tolerance in vivo. J Immunol 174(4):2046–2053
Preynat-Seauve O, Contassot E, Schuler P, Piguet V, French LE, Huard B (2007) Extralymphatic tumors prepare draining lymph nodes to invasion via a T-cell cross-tolerance process. Cancer Res 67(10):5009–5016
Zhou G, Garofalo S, Mukhopadhyay K, Lefebvre V, Smith CN, Eberspaecher H, de Crommbrugghe B (1995) A 182 bp fragment of the mouse pro alpha1(II) collagen is sufficient to direct chondrocyte expression in transgenic mice. J Cell Sci 108(Pt12):3677–3684
Osman GE, Cheunsuk S, Allen SE, Chi E, Liggit HD, Hood LE, Ladiges WC (1998) Expression of a type II collagen-specific TCR transgene accelerates the onset of arthritis in mice. Int Immunol 10(11):1613–1622
Wells AD, Gumundsdottir H, Turka LA (1997) Following the fate of individual T cells throughout activation and clonal expansion. Signals from T-cell receptor and CD28 differentially regulate the induction and duration of a proliferative response. J Clin Invest 100(12):3173–3183
Mintern JD, Davey GM, Belz GT, Carbone FR, Heath WR (2002) Cutting edge: precursor frequency affects the helper dependence of cytotoxic T cells. J Immunol 168(3):977–980
Acknowledgements
Our experience in adoptive T-cell transfer assays has been possible with the financial support from the Swiss National Science Foundation, the Swiss Cancer League, and the Geneva Cancer League.
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Bertrand, H. (2014). Unraveling Autoimmunity with the Adoptive Transfer of T Cells from TCR-Transgenic Mice. In: Shiozawa, S. (eds) Arthritis Research. Methods in Molecular Biology, vol 1142. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0404-4_5
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DOI: https://doi.org/10.1007/978-1-4939-0404-4_5
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