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Use of the Dynamic TIM-1 Model for an In-Depth Understanding of the Survival and Virulence Gene Expression of Shiga Toxin-Producing Escherichia coli in the Human Stomach and Small Intestine

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Shiga Toxin-Producing E. coli

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2291))

Abstract

Due to obvious ethical and technical reasons, it remains very difficult to evaluate the survival and expression of virulence genes of food-borne pathogens, such as Shiga toxin-producing Escherichia coli (STEC) in the human gastrointestinal tract. Here, we describe the use of the dynamic TNO (Toegepast Natuurwetenschappelijk Onderzoek) gastrointestinal model (TIM-1) as a powerful in vitro tool to obtain the kinetics of STEC survival by plate counting, the regulation of major virulence genes by RT-qPCR, and the production of Shiga toxins by ELISA, in the human stomach and small intestine. The gut model was adapted in order that in vitro digestions were performed both under adult and child digestive conditions, specific at risk populations for STEC infections.

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Author notes

  1. Ophélie Uriot and Sandrine Chalancon contributed equally to this work.

Authors and Affiliations

  1. Microbiology Digestive Environment and Health, UMR UCA-INRA 454 MEDIS, Université Clermont Auvergne, Clermont-Ferrand, France

    Ophélie Uriot, Sandrine Chalancon, Carine Mazal, Lucie Etienne-Mesmin, Sylvain Denis & Stéphanie Blanquet-Diot

Authors
  1. Ophélie Uriot
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  2. Sandrine Chalancon
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  3. Carine Mazal
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  4. Lucie Etienne-Mesmin
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  5. Sylvain Denis
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  6. Stéphanie Blanquet-Diot
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Corresponding author

Correspondence to Stéphanie Blanquet-Diot .

Editor information

Editors and Affiliations

  1. University of East Anglia, Norwich Medical School, Norwich, UK

    Stephanie Schüller

  2. National Reference Laboratory for E. coli and Shigellae, National Institute of Public Health, Prague, Czech Republic

    Martina Bielaszewska

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Uriot, O., Chalancon, S., Mazal, C., Etienne-Mesmin, L., Denis, S., Blanquet-Diot, S. (2021). Use of the Dynamic TIM-1 Model for an In-Depth Understanding of the Survival and Virulence Gene Expression of Shiga Toxin-Producing Escherichia coli in the Human Stomach and Small Intestine. In: Schüller, S., Bielaszewska, M. (eds) Shiga Toxin-Producing E. coli . Methods in Molecular Biology, vol 2291. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1339-9_14

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  • DOI: https://doi.org/10.1007/978-1-0716-1339-9_14

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1338-2

  • Online ISBN: 978-1-0716-1339-9

  • eBook Packages: Springer Protocols

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