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A Method for the Establishment and Characterization of Mouse Lung Adenocarcinoma Cell Lines that Mimic Traits of Human Adenocarcinomas

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Lung Cancer

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2279))

Abstract

Lung adenocarcinoma (LADC) is the leading cause of cancer death worldwide and is largely inflicted by carcinogens contained in tobacco smoke. The generation of cell lines mimicking traits of human LADC will profoundly advance our understanding of the pathobiology of the disease, as they offer an easy and valuable tool to study the cellular and molecular aspects of carcinogenesis. Here we describe a detailed protocol for the generation of such cell lines, following the exposure of experimental mouse strains to different tobacco carcinogens and isolation of the resulting lung tumors.

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Acknowledgments

The work was supported by European Research Council 2010 Starting Independent Investigator and 2015 Proof of Concept Grants (grant numbers 260524 and 679345 respectively; to G.T.S.).

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Authors and Affiliations

  1. Laboratory for Molecular Respiratory Carcinogenesis, Department of Physiology, Faculty of Medicine, University of Patras, Rio, Greece

    Magda Spella, Ioannis Lilis & Georgios T. Stathopoulos

  2. Comprehensive Pneumology Center (CPC) and Institute for Lung Biology and Disease (iLBD), University Hospital, Ludwig-Maximilians University and Helmholtz Center Munich, Member of the German Center for Lung Research (DZL), Munich, Germany

    Georgios T. Stathopoulos

Authors
  1. Magda Spella
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  2. Ioannis Lilis
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  3. Georgios T. Stathopoulos
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Corresponding author

Correspondence to Georgios T. Stathopoulos .

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Editors and Affiliations

  1. Biochemistry and Cancer Biology Divisions, Ponce Health Sciences University-Ponce Research Institute, Ponce, Puerto Rico

    Pedro G. Santiago-Cardona

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Spella, M., Lilis, I., Stathopoulos, G.T. (2021). A Method for the Establishment and Characterization of Mouse Lung Adenocarcinoma Cell Lines that Mimic Traits of Human Adenocarcinomas. In: Santiago-Cardona, P.G. (eds) Lung Cancer. Methods in Molecular Biology, vol 2279. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1278-1_14

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  • DOI: https://doi.org/10.1007/978-1-0716-1278-1_14

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1277-4

  • Online ISBN: 978-1-0716-1278-1

  • eBook Packages: Springer Protocols

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