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High-Dimensional Phenotyping of Human Myeloid-Derived Suppressor Cells/Tumor-Associated Macrophages in Tissue by Mass Cytometry

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Myeloid-Derived Suppressor Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2236))

Abstract

Myeloid-derived suppressor cells (MDSCs) and tumor-associated macrophages (TAMs) are heterogeneous cells that share myeloid markers and are not easily distinguishable in human tumors due to their lack of specific markers. These cells are a major player in the tumor microenvironment and are involved in the prognosis and physiopathology of various tumors. Here is presented a scheme to decipher these cells by mass cytometry.

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Acknowledgments

Juliette Ferrant is recipient of a fellowship from the FHU CAMIn (Federation Hospitalo-Universitaire Cancer Microenvironnement et Innovation). Guillaume Manson and Steve Genebrier were funded by a Master 2 fellowship from the Fondation ARC. This work was supported by grants from the Agence Nationale de la Recherche (ANR-17-CE15-0015 StroMAC) and Fondation ARC (PGA1 RF20190208534).

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Authors and Affiliations

  1. Institut national de la santé et de la recherche médicale, Unité Mixte de Recherche U1236, Université Rennes 1, Etablissement Français du Sang Bretagne, Rennes, France

    Juliette Ferrant, Simon Le Gallou, Guillaume Manson, Steve Genebrier, Frederic Mourcin, Karin Tarte & Mikael Roussel

  2. Centre Hospitalier Universitaire de Rennes, Pôle Biologie, Rennes, France

    Simon Le Gallou, Karin Tarte & Mikael Roussel

Authors
  1. Juliette Ferrant
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  2. Simon Le Gallou
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  3. Guillaume Manson
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  4. Steve Genebrier
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  5. Frederic Mourcin
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  6. Karin Tarte
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  7. Mikael Roussel
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Corresponding author

Correspondence to Mikael Roussel .

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Editors and Affiliations

  1. Experimental and Translational Research, Department of Otorhinolaryngology, University Hospital Essen, West German Cancer Center, Essen, Germany

    Sven Brandau

  2. Institute of Immunology, Friedrich-Loeffler-Institut, Greifswald-Insel Riems, Germany

    Anca Dorhoi

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Ferrant, J. et al. (2021). High-Dimensional Phenotyping of Human Myeloid-Derived Suppressor Cells/Tumor-Associated Macrophages in Tissue by Mass Cytometry. In: Brandau, S., Dorhoi, A. (eds) Myeloid-Derived Suppressor Cells. Methods in Molecular Biology, vol 2236. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1060-2_6

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  • DOI: https://doi.org/10.1007/978-1-0716-1060-2_6

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1059-6

  • Online ISBN: 978-1-0716-1060-2

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