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Intravital 2-Photon Microscopy of Diverse Cell Types in the Murine Tibia

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Myeloid-Derived Suppressor Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2236))

Abstract

Intravital imaging allows the visualization of fluorescently labeled structures like cells, blood flow, and pathogens in a living organism. Nowadays, numerous methods for imaging in several organs are available. In this chapter, we present a method for intravital 2-photon microscopy of the murine tibial bone marrow. It enables the observation of hematopoietic cells including cells of the innate and adaptive immune system under physiological conditions. Motility analyses within this complex environment led to insights into their migratory potential as well as their interactions with other cells or blood vessels.

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Acknowledgments

This work was partly funded by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation)—GU769/13-1 (KFO 337), GU769/10-1, GU769/11-1 and GU769/15-1 (FOR 2879) to M.G.

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Author notes

  1. Anja Hasenberg, Lucas Otto, and Matthias Gunzer contributed equally to this work.

Authors and Affiliations

  1. Institute for Experimental Immunology and Imaging, University Hospital, University Duisburg-Essen, Essen, Germany

    Anja Hasenberg, Lucas Otto & Matthias Gunzer

  2. Institute for Virology, University Hospital, University Duisburg-Essen, Essen, Germany

    Lucas Otto

  3. Leibniz-Institut für Analytische Wissenschaften – ISAS -e.V, Dortmund, Germany

    Matthias Gunzer

Authors
  1. Anja Hasenberg
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  2. Lucas Otto
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  3. Matthias Gunzer
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Corresponding author

Correspondence to Anja Hasenberg .

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Editors and Affiliations

  1. Experimental and Translational Research, Department of Otorhinolaryngology, University Hospital Essen, West German Cancer Center, Essen, Germany

    Sven Brandau

  2. Institute of Immunology, Friedrich-Loeffler-Institut, Greifswald-Insel Riems, Germany

    Anca Dorhoi

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Hasenberg, A., Otto, L., Gunzer, M. (2021). Intravital 2-Photon Microscopy of Diverse Cell Types in the Murine Tibia. In: Brandau, S., Dorhoi, A. (eds) Myeloid-Derived Suppressor Cells. Methods in Molecular Biology, vol 2236. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1060-2_15

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  • DOI: https://doi.org/10.1007/978-1-0716-1060-2_15

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1059-6

  • Online ISBN: 978-1-0716-1060-2

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