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Quantitative Analysis of Integrin Trafficking

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The Integrin Interactome

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2217))

Abstract

The last two decades of research into integrin trafficking has revealed fascinating insight into the function of integrin receptors, particularly in the context of cell invasion and migration in cancer. Deregulation in the trafficking pathways of integrin receptors contributes to a variety of pathological conditions including cancer, and in fact, altered endocytic trafficking of these receptors has been shown to drive transformation and tumor progression. Being able to experimentally measure integrin internalization, recycling and cell surface levels are vital for determining the role integrins play in health and disease. Surface-labeling based endocytic trafficking assays provide a way to experimentally measure changes in the rate of internalization of cell surface proteins, and the recycling of internalized proteins back to the cell surface, with high accuracy. This chapter will focus on quantitative approaches based on cell surface biotinylation and capture ELISA to measure endocytosis, recycling, and cell surface levels of integrin receptors.

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Author information

Authors and Affiliations

  1. Wellcome Trust Centre for Cell-Matrix Research, Division of Cell Matrix Biology and Regenerative Medicine, School of Biological Sciences, University of Manchester, Manchester, UK

    Enoir Farage & Patrick T. Caswell

Authors
  1. Enoir Farage
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  2. Patrick T. Caswell
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Correspondence to Patrick T. Caswell .

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Editors and Affiliations

  1. Molecular Mechanisms Program, Centro de Investigación del Cáncer and Instituto de Biología Molecular y Celular del Cáncer, Consejo Superior de Investigaciones Cientóficas (CSIC)-University of Salamanca, Salamanca, Spain

    Miguel Vicente-Manzanares

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Farage, E., Caswell, P.T. (2021). Quantitative Analysis of Integrin Trafficking. In: Vicente-Manzanares, M. (eds) The Integrin Interactome. Methods in Molecular Biology, vol 2217. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0962-0_14

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  • DOI: https://doi.org/10.1007/978-1-0716-0962-0_14

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0961-3

  • Online ISBN: 978-1-0716-0962-0

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