Abstract
Nanopore technology enables the detection and analysis of single protein molecules. The technique measures the ionic current passing through a single pore inserted in an electrically insulating membrane. The translocation of the protein molecule through the pore causes a modulation of the ionic current. Analysis of the ionic current reveals the biophysics of co-translocational unfolding and may be used to infer the amino acid sequence and posttranslational modifications of the molecule.
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Acknowledgements
D.R.-L. is a recipient of a RamĆ³n y Cajal Fellowship (RYC-2013-12799). D.R.-L. was funded by MINECO grants BIO2017-88946-R and BFU2016-81754-ERC (FEDER funds).
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Celaya, G., Rodriguez-Larrea, D. (2021). Oligonucleotide-Directed Protein Threading Through a Rigid Nanopore. In: Fahie, M.A. (eds) Nanopore Technology. Methods in Molecular Biology, vol 2186. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0806-7_10
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DOI: https://doi.org/10.1007/978-1-0716-0806-7_10
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