Abstract
In arbuscular mycorrhizal symbiosis, the belowground mycelium that develops into the soil, not only provides extensive pathways for nutrient fluxes, the occupation of different niches, and dispersal of propagules, but also has strong influences upon biogeochemical cycling. By providing a valuable overview of expression changes of most proteins, shotgun proteomics can help decipher key metabolic pathways involved in the functioning of fungal mycelia. In this protocol, we describe the combination of extra-radical mycelium growth systems with gel-based extraction of fungal peptides amenable for shotgun protein profiling, which allows gaining information about the extra-radical proteome.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Bago B, Azcón-Aguilar C, Piché Y (1998) Architecture and developmental dynamics of the external mycelium of the arbuscular mycorrhizal fungus Glomus intraradices grown under monoxenic conditions. Mycologia 90:52–62
Bücking H, Liepold E, Ambilwade P (2012) The role of the mycorrhizal symbiosis in nutrient uptake of plants and the regulatory mechanisms underlying these transport processes. Plant Sci 4:108–132
Leake J, Johnson D, Donnelly D et al (2004) Networks of power and influence: the role of mycorrhizal mycelium in controlling plant communities and agroecosystem functioning. Can J Bot 82:1016–1045
Walder F, Niemann H, Natarajan M et al (2012) Mycorrhizal networks: common goods of plants shared under unequal terms of trade. Plant Physiol 159:789–797
Fellbaum CR, Mensah JA, Cloos AJ et al (2014) Fungal nutrient allocation in common mycorrhizal networks is regulated by the carbon source strength of individual host plants. New Phytol 203:646–656
Gorzelak MA, Asay AK, Pickles BJ, Simard SW (2015) Inter-plant communication through mycorrhizal networks mediates complex adaptive behaviour in plant communities. AoB Plants 7:plv050
Johson D, Gilbert L (2015) Interplant signalling through hyphal networks. New Phytol 205:1448–1453
Hanssen JF, Thingstad TF, Goksoyr J (1974) Evaluation of hvphal lengths and fungal biomass in soil by a membrane filter technique. Oikos 25:102–107
Li XL, George E, Marschner H (1991) Phosphorus depletion and pH decrease at the root-soil and hyphae soil interfaces of VA mycorrhizal white clover fertilized with ammonium. New Phytol 119:397–404
St-Arnaud M, Hamel C, Vimard B et al (1996) Enhanced hyphal growth and spore production of the arbuscular mycorrhizal fungus Glomus intraradices in an in vitro system in the absence of host roots. Mycol Res 100:328–332
Kuhn H, Küster H, Requena N (2010) Membrane steroid-binding protein 1 induced by a diffusible fungal signal is critical for mycorrhization in Medicago truncatula. New Phytol 185:716–733
Gutteridge A, Pir P, Castrillo JI et al (2010) Nutrient control of eukaryote cell growth: a systems biology study in yeast. BMC Biol 8:68
Plaxton WC, Tran HT (2011) Metabolic adaptations of phosphate-starved plants. Plant Physiol 156:1006–1015
Arif W, Datar G, Kalsotra A (2017) Intersections of post-transcriptional gene regulatory mechanisms with intermediary metabolism. Biochim Biophys Acta 1860:349–362
Alexova R, Millar AH (2013) Proteomics of phosphate use and deprivation in plants. Proteomics 13:609–623
Haynes PA, Roberts TH (2007) Subcellular shotgun proteomics in plants: looking beyond the usual suspects. Proteomics 7:2963–2975
Laemmli UK, Amos LA, Klug A (1970) Correlation between structural transformation and cleavage of the major head protein of T4 bacteriophage. Cell 7:191–203
Zhang Q, Yang R, Tang J et al (2010) Positive feedback between mycorrhizal fungi and plants influences plant invasion success and resistance to invasion. PLoS One 5:e12380
Bradford MM (1976) A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254
Ramagli LS, Rodriguez LW (1985) Quantification of microgram amounts of protein in two-dimensional polyacrylamide gel electrophoresis sample buffer. Electrophoresis 6:559–563
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2020 Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Recorbet, G., Courty, PE., Wipf, D. (2020). Recovery of Extra-Radical Fungal Peptides Amenable for Shotgun Protein Profiling in Arbuscular Mycorrhizae. In: Ferrol, N., Lanfranco, L. (eds) Arbuscular Mycorrhizal Fungi. Methods in Molecular Biology, vol 2146. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0603-2_17
Download citation
DOI: https://doi.org/10.1007/978-1-0716-0603-2_17
Published:
Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-0602-5
Online ISBN: 978-1-0716-0603-2
eBook Packages: Springer Protocols