Abstract
Bioluminescence is a rapid and cost-saving technology that enables monitoring of bacteria in real time in animal infection models. Here, we report a method for labeling GAS with a set of plasmids we have developed and deposited with Addgene. These plasmids can be used to either integrate firefly luciferase (Ffluc) or red-shifted firefly luciferase (FflucRT) into the GAS genome or to introduce the reporters on plasmids which have been stabilized with a toxin–antitoxin system to prevent loss of plasmid in the absence of antibiotic selection.
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Acknowledgments
We thank Andreas Podbielski (University of Rostock, Germany) for sending us the pFW11 vector, Nobuhiko Okada (Kitasato University, Tokyo, Japan) for providing the pLZ12Km2 vector, and Urszula Zielenkiewicz (Polish Academy of Sciences, Warsaw, Poland) for sending the pBT286 plasmid containing the TA cassette. This project was supported by a research grant from the Health Research Council (HRC) New Zealand.
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Loh, J.M.S., Soh, K.Y., Proft, T. (2020). Generation of Bioluminescent Group A Streptococcus for Biophotonic Imaging. In: Proft, T., Loh, J. (eds) Group A Streptococcus. Methods in Molecular Biology, vol 2136. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0467-0_6
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DOI: https://doi.org/10.1007/978-1-0716-0467-0_6
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