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Detection of Streptococcus pyogenes Virulence Factors

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Group A Streptococcus

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2136))

Abstract

Streptococcus pyogenes encodes multiple virulence factors and their presence is often related to the severity of the disease. We designed the system of four low-volume multiplex PCR reactions to detect genes encoding 20 virulence factors: spd3, sdc, sdaB, sdaD, speB, spyCEP, scpA, mac, sic, speL, speK, speM, speC, speI, speA, speH, speG, speJ, smeZ, and ssa. Classification of strains based on the virulence factors absence or presence correlates with PFGE MLST and emm typing results. The typing/detection system is fast and cost-effective, can be used to detect GAS virulence factors and as a rapid tool to effectively differentiate between strains.

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Acknowledgments

The work was supported by grants 2017/27/B/NZ7/00040 and 2018/29/B/NZ6/00624 to I.S.

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Authors and Affiliations

  1. Department of Drug Biotechnology and Bioinformatics, National Medicines Institute, Warszawa, Poland

    Aleksandra Kozińska & Izabela Sitkiewicz

Authors
  1. Aleksandra Kozińska
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  2. Izabela Sitkiewicz
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Editor information

Editors and Affiliations

  1. School of Medical Sciences, The University of Auckland, Auckland, New Zealand

    Thomas Proft

  2. School of Medical Sciences, The University of Auckland, Auckland, New Zealand

    Jacelyn M. S. Loh

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Kozińska, A., Sitkiewicz, I. (2020). Detection of Streptococcus pyogenes Virulence Factors. In: Proft, T., Loh, J. (eds) Group A Streptococcus. Methods in Molecular Biology, vol 2136. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0467-0_1

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  • DOI: https://doi.org/10.1007/978-1-0716-0467-0_1

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0466-3

  • Online ISBN: 978-1-0716-0467-0

  • eBook Packages: Springer Protocols

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