Abstract
Effective RNA delivery strategies for primary human monocytes and dendritic cells (DCs) are useful tools for both basic research and cancer immunotherapy applications. Compared to viral delivery, electroporation is a relatively safe and simple technique that has been established for most immune cells. This chapter describes the feasibility of introducing small interfering RNAs into human primary monocytes and DCs using either nucleofection or standard electroporation techniques. DC cancer vaccines that integrate siRNA targeting relevant DC-intrinsic immunosuppressive signals induced robust and durable anti-tumor immune responses.
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Acknowledgments
This work was supported by a grant from the Norwegian Cancer Society. The author thanks Flatekval G Furset for performing the experiments described in this chapter.
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Sioud, M. (2020). Optimized siRNA Delivery into Primary Immune Cells Using Electroporation. In: Sioud, M. (eds) RNA Interference and CRISPR Technologies. Methods in Molecular Biology, vol 2115. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0290-4_7
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DOI: https://doi.org/10.1007/978-1-0716-0290-4_7
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