Abstract
In vitro differentiation of naïve CD4+ T cells into effector and regulatory subsets offers a means to acquire large numbers of relatively homogeneous cell populations for experimentation. However, culture systems for T cell differentiation described in the literature vary widely in efficiency and complexity, limiting their comparison across studies. Here, we present a standardized and robust method for the isolation and in vitro differentiation of six CD4+ T cell subsets from mouse naïve T cells.
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Acknowledgments
This work was supported by NIH grant R01 GM115474 to M.C.
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Espinosa, J.R., Wheaton, J.D., Ciofani, M. (2020). In Vitro Differentiation of CD4+ T Cell Effector and Regulatory Subsets. In: Liu, C. (eds) T-Cell Receptor Signaling. Methods in Molecular Biology, vol 2111. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0266-9_7
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DOI: https://doi.org/10.1007/978-1-0716-0266-9_7
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-0265-2
Online ISBN: 978-1-0716-0266-9
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