Abstract
Ubiquitination is a crucial component of many immune processes. While ubiquitin-mediated degradation is essential to T cell activation via T cell receptor signaling, the specific E3 ligases and substrates involved are not well-understood. Here, we describe a strategy integrating RNA, protein, and posttranslational modification datasets to identify targets of ubiquitin-mediated degradation. When integrated, these assays can provide broad insight into how this posttranslational modification regulates protein function and influences T cell biology.
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Field, N.S., O’Leary, C.E., Dybas, J.M., Ding, H., Oliver, P.M. (2020). An Integrated Strategy for Identifying Targets of Ubiquitin-Mediated Degradation in CD4+ T Cells. In: Liu, C. (eds) T-Cell Receptor Signaling. Methods in Molecular Biology, vol 2111. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0266-9_19
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DOI: https://doi.org/10.1007/978-1-0716-0266-9_19
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