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Primary T-Cell Transduction to Study Follicular Helper T-Cell Differentiation

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T-Cell Receptor Signaling

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2111))

Abstract

Follicular helper T (Tfh) cells constitute a specialized CD4+ T-cell subset that localizes in close proximity to B cells and are essential in the production of high-affinity, class-switched antibodies, and their dysregulations are also involved in autoimmune diseases. Modulating gene expression patterns in primary T cells is an important approach to understanding Tfh cell differentiation and function. In this chapter, we describe a protocol to evaluate Tfh cell differentiation with OT-II TCR transgenic T cells by retrovirally transducing gene of interest. This protocol adopts the recombinant retrovirus-based transduction of primary CD4+ T cells, and it also includes procedures for adoptive transfer, immunization, and flow cytometry analysis.

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Authors and Affiliations

  1. Department of Immunology, Nanjing Medical University, Nanjing, Jiangsu, China

    Yang Zhang, Xuehui Long & Xiaoming Wang

Authors
  1. Yang Zhang
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  2. Xuehui Long
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  3. Xiaoming Wang
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Corresponding author

Correspondence to Xiaoming Wang .

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Editors and Affiliations

  1. Department of Pathogen Biology, Huazhong University of Science and Technology, Wuhan, China

    Chaohong Liu

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Zhang, Y., Long, X., Wang, X. (2020). Primary T-Cell Transduction to Study Follicular Helper T-Cell Differentiation. In: Liu, C. (eds) T-Cell Receptor Signaling. Methods in Molecular Biology, vol 2111. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0266-9_10

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  • DOI: https://doi.org/10.1007/978-1-0716-0266-9_10

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0265-2

  • Online ISBN: 978-1-0716-0266-9

  • eBook Packages: Springer Protocols

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