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Protocol for the Application of Bioluminescence Full-Cell Bioreporters for Monitoring of Terrestrial Bioremediation

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Hydrocarbon and Lipid Microbiology Protocols

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Abstract

Microbial full-scale bioreporters are associated with a variety of names that include biosensors, bio-indicators and bio-reactive agents. The role of such microbial agents is to respond to the bioavailable fraction of a given analyte under “near environmental conditions”. Making use of appropriate assays with relevant and biologically compatible extraction procedures means that such techniques can be applied to develop site specific risk and hazard assessments, an appraisal of constraints inhibiting biodegradation and a prediction of potential for biodegradation. The effectiveness of an assay requires: (1) the comprehensive characterisation of the marker gene (the reporter gene) and the isolate, (2) the collection of an environmentally relevant sample in a suitable matrix and (3) a technique to integrate the bioreporters with the sample to generate focussed and relevant data. This technology is ideally placed for high throughput, rapid screening of samples from a range of environmental matrices.

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Author information

Authors and Affiliations

  1. Institute of Biological and Environmental Sciences, University of Aberdeen, St. Machar Drive, Aberdeen, AB24 3UU, Scotland, UK

    Sarah B. Sinebe, Ogonnaya I. Iroakasi & Graeme I. Paton

  2. Remedios Ltd, Brathens Eco-Business Park, Hill of Brathens, Glassel, Banchory, AB31 4BW, Scotland, UK

    Graeme I. Paton

Authors
  1. Sarah B. Sinebe
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  2. Ogonnaya I. Iroakasi
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  3. Graeme I. Paton
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Corresponding author

Correspondence to Sarah B. Sinebe .

Editor information

Editors and Affiliations

  1. School of Biological Sciences, University of Essex Dept. Biological Sciences, Colchester, Essex, United Kingdom

    Terry J. McGenity

  2. Institute of Microbiology, Technical University Braunschweig, Braunschweig, Germany

    Kenneth N. Timmis

  3. Department of Biology, University of the Balearic Islands and Mediterranean Institute for Advanced Studies (IMEDEA, UIB-CSIC), Palma de Mallorca, Spain

    Balbina Nogales

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© 2016 Springer-Verlag Berlin Heidelberg

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Sinebe, S.B., Iroakasi, O.I., Paton, G.I. (2016). Protocol for the Application of Bioluminescence Full-Cell Bioreporters for Monitoring of Terrestrial Bioremediation. In: McGenity, T., Timmis, K., Nogales, B. (eds) Hydrocarbon and Lipid Microbiology Protocols. Springer Protocols Handbooks. Springer, Berlin, Heidelberg. https://doi.org/10.1007/8623_2016_226

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  • DOI: https://doi.org/10.1007/8623_2016_226

  • Published:

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-662-53113-6

  • Online ISBN: 978-3-662-53115-0

  • eBook Packages: Springer Protocols

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