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Isolation of Glycoprotein Bioemulsifiers Produced by Marine Bacteria

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Hydrocarbon and Lipid Microbiology Protocols

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Abstract

High-molecular-weight (HMW) surface-active agents (biosurfactants and bioemulsifiers) are produced by many different types of bacteria and comprise proteins, glycoproteins, lipoproteins, polysaccharides, lipopolysaccharides or complexes containing any combination of these structural types. The attraction of HMW glycoprotein bioemulsifiers as ingredients in food and drink formulations, for example, has increased in recent years, especially those produced by marine bacteria, as these amphiphilic macromolecules can offer improved emulsifying and emulsion stability properties. Marine bacteria are a largely untapped source for these types of molecules, and unlike those produced by terrestrial bacteria and microalgae, marine bacterial glycoproteins are often highly negatively charged (polyanionic) which endows these macromolecules with potential multifunctional properties. The polyanionic nature and molecular-weight heterogeneity of these types of molecules require careful attention to optimising their isolation from complex media. This chapter provides a detailed description for optimising the isolation/separation of amphiphilic polyanionic glycoprotein bioemulsifiers produced by marine bacteria.

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Author information

Authors and Affiliations

  1. School of Life Sciences, Centre for Marine Biodiversity and Biotechnology, Heriot-Watt University, Edinburgh, EH14 4AS, UK

    Tony Gutierrez

  2. School of Biomedical Sciences, Faculty of Life and Health Sciences, University of Ulster, Coleraine, BT52 1SA, Northern Ireland, UK

    Ibrahim M. Banat

Authors
  1. Tony Gutierrez
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  2. Ibrahim M. Banat
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Corresponding author

Correspondence to Tony Gutierrez .

Editor information

Editors and Affiliations

  1. School of Biological Sciences, University of Essex, Colchester, Essex, United Kingdom

    Terry J. McGenity

  2. Institute of Microbiology, Technical University Braunschweig, Braunschweig, Germany

    Kenneth N. Timmis

  3. Dept. Biology, University of the Balearic Islands and University of the Balearic Islands and Mediterranean Institute for Advanced Studies (IMEDEA, UIB-CSIC)", Palma de Mallorca, Spain

    Balbina Nogales

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© 2015 Springer-Verlag Berlin Heidelberg

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Gutierrez, T., Banat, I.M. (2015). Isolation of Glycoprotein Bioemulsifiers Produced by Marine Bacteria. In: McGenity, T., Timmis, K., Nogales , B. (eds) Hydrocarbon and Lipid Microbiology Protocols . Springer Protocols Handbooks. Springer, Berlin, Heidelberg. https://doi.org/10.1007/8623_2015_128

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  • DOI: https://doi.org/10.1007/8623_2015_128

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  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-662-49135-5

  • Online ISBN: 978-3-662-49137-9

  • eBook Packages: Springer Protocols

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