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Tumor Targeting by RGD-Grafted PLGA-Based Nanotheranostics Loaded with Paclitaxel and Superparamagnetic Iron Oxides

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Integrin Targeting Systems for Tumor Diagnosis and Therapy

Part of the book series: Methods in Pharmacology and Toxicology ((MIPT))

Abstract

Theranostic nanoparticles have the potential to revolutionize cancer diagnosis and therapy. Many groups have demonstrated differential levels of tumor growth between tumors treated by targeted or untargeted nanoparticles; however, only few have shown in vivo efficacy in both therapeutic and diagnostic approach. Herein, we first develop and characterize dual-paclitaxel (PTX)/superparamagnetic iron oxide (SPIO)-loaded PLGA-based nanoparticles grafted with the RGD peptide, for a theranostic purpose. Second, we compare in vivo different strategies in terms of targeting capabilities: (1) passive targeting via the EPR effect, (2) active targeting of αvβ3 integrin via RGD grafting, (3) magnetic guidance via a magnet placed on the tumor, and (4) the combination of the magnetic guidance and the active targeting of αvβ3 integrin. In this chapter, we present the general flowchart applied for this project: (1) the polymer and SPIO synthesis, (2) the physicochemical characterization of the nanoparticles, (3) the magnetic properties of the nanoparticles, and (4) the in vivo evaluation of the nanoparticles for their therapeutic and diagnosis purposes. We employ the electron spin resonance spectroscopy and magnetic resonance imaging to both quantify and visualize the accumulation of theranostic nanoparticles into the tumors.

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Acknowledgment

This work is supported by grants from the Université catholique de Louvain (F.S.R.) and Fonds National de la Recherche Scientifique (F.R.S.-F.N.R.S.). F. Danhier is a Postdoctoral F.R.S.-F.N.R.S. Research Fellow. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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Authors and Affiliations

  1. Louvain Drug Research Institute, Advanced Drug delivery and Biomaterials, Université catholique de Louvain, Avenue Mounier, B1 73.12, 1200, Brussels, Belgium

    Fabienne Danhier, Nathalie Schleich & Véronique Préat

  2. Louvain Drug Research Institute, Laboratory of Biomedical Magnetic Resonance, Université catholique de Louvain, Avenue Mounier, B1 73.08, 1200, Brussels, Belgium

    Pierre Danhier, Chrystelle Po & Bernard Gallez

  3. Department of General, Organic, and Biomedical Chemistry, NMR and Molecular Imaging Laboratory, Université de Mons, 7000, Mons, Belgium

    Sophie Laurent

  4. Center for Education and Research on Macromolecule, Université de Liège, Sart Tilman B6, 4000, Liège, Belgium

    Pierre Sibret & Christine Jérôme

  5. Unité de Chimie organique et médicinale, Université catholique de Louvain, Louis Pasteur, 1, 1348, Louvain-la-Neuve, Belgium

    Vincent Poucelle

Authors
  1. Fabienne Danhier
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  2. Pierre Danhier
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  3. Nathalie Schleich
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  4. Chrystelle Po
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  5. Sophie Laurent
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  6. Pierre Sibret
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  7. Christine Jérôme
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  8. Vincent Poucelle
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  9. Bernard Gallez
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  10. Véronique Préat
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Corresponding author

Correspondence to Véronique Préat .

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Editors and Affiliations

  1. Department of Gastroenterology and Hepatology, UniversitätsSpital Zürich, Zürich, Switzerland

    Eleonora Patsenker

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Danhier, F. et al. (2015). Tumor Targeting by RGD-Grafted PLGA-Based Nanotheranostics Loaded with Paclitaxel and Superparamagnetic Iron Oxides. In: Patsenker, E. (eds) Integrin Targeting Systems for Tumor Diagnosis and Therapy. Methods in Pharmacology and Toxicology. Humana Press, New York, NY. https://doi.org/10.1007/7653_2015_43

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  • DOI: https://doi.org/10.1007/7653_2015_43

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7443-6

  • Online ISBN: 978-1-4939-7445-0

  • eBook Packages: Springer Protocols

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