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Whole-Mount Immunofluorescent Staining Coupled to Multicolor Lineage Tracing Model for Analyzing the Spatiotemporal Organization of Epidermal Stem Cells

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Skin Stem Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1879))

Abstract

An overall three-dimensional picture of the distribution of epidermal cells at a given time point is of importance to better characterize epidermal progenitors. We introduce a whole-mount immunofluorescent staining coupled to a multicolor lineage tracing model for analyzing the spatiotemporal organization of epidermal stem cells. Laser scanning confocal microscopy with multiple labelling allows for robust imaging of dorsal skin with excellent resolution.

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References

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Acknowledgements

This work was supported by the Australian Research Council Discovery Project DP130104777 and the National Health and Medical Research Council project grant 1145350.

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Authors and Affiliations

  1. The University of Queensland, UQ Diamantina Institute, Translational Research Institute, Brisbane, QLD, Australia

    Edwige Roy & Kiarash Khosrotehrani

Authors
  1. Edwige Roy
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  2. Kiarash Khosrotehrani
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Corresponding author

Correspondence to Edwige Roy .

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Editors and Affiliations

  1. Ottawa, ON, Canada

    Kursad Turksen

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© 2018 Springer Science+Business Media New York

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Roy, E., Khosrotehrani, K. (2018). Whole-Mount Immunofluorescent Staining Coupled to Multicolor Lineage Tracing Model for Analyzing the Spatiotemporal Organization of Epidermal Stem Cells. In: Turksen, K. (eds) Skin Stem Cells. Methods in Molecular Biology, vol 1879. Humana Press, New York, NY. https://doi.org/10.1007/7651_2018_165

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  • DOI: https://doi.org/10.1007/7651_2018_165

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8869-3

  • Online ISBN: 978-1-4939-8870-9

  • eBook Packages: Springer Protocols

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