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Isolation and Enrichment of Newborn and Adult Skin Stem Cells of the Interfollicular Epidermis

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Skin Stem Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1879))

Abstract

The interfollicular epidermis regenerates from a heterogeneous population of basal cells undergoing either self-renewal or terminal differentiation, thereby balancing cell loss in tissue turnover or in wound repair. In this chapter, we describe a reliable and simple method for isolating interfollicular epithelial stem cells from the skin of newborn mice or from tail and ear skin of adult mice using fluorescence-activated cell sorting (FACS). We also provide a detailed protocol for culturing interfollicular epidermal stem cells and to assess their proliferative potential and self-renewing ability. These techniques are useful for directly evaluating epidermal stem cell function in normal mice under different conditions or in genetically modified mouse models.

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Acknowledgments

This work was supported by the Telethon grant GGP16235 and the Italian Association for Cancer Research (AIRC IG2011-N.11369).

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Authors and Affiliations

  1. Department of Biology, University of Naples Federico II, Napoli, Italy

    Stefano Sol, Dario Antonini & Caterina Missero

  2. CEINGE Biotecnologie Avanzate (Center for Genetic Engineering), Napoli, Italy

    Stefano Sol, Dario Antonini & Caterina Missero

Authors
  1. Stefano Sol
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  2. Dario Antonini
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  3. Caterina Missero
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Corresponding author

Correspondence to Caterina Missero .

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Editors and Affiliations

  1. Ottawa, ON, Canada

    Kursad Turksen

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Sol, S., Antonini, D., Missero, C. (2018). Isolation and Enrichment of Newborn and Adult Skin Stem Cells of the Interfollicular Epidermis. In: Turksen, K. (eds) Skin Stem Cells. Methods in Molecular Biology, vol 1879. Humana Press, New York, NY. https://doi.org/10.1007/7651_2018_131

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  • DOI: https://doi.org/10.1007/7651_2018_131

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8869-3

  • Online ISBN: 978-1-4939-8870-9

  • eBook Packages: Springer Protocols

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