Abstract
One of the major issues concerning human skeletal muscle progenitor cells is represented by the efficient isolation and in vitro expansion of cells retaining the ability to proliferate, migrate and differentiate once transplanted. Here we describe a method (1) effective in obtaining human muscle precursor cells both from fresh and frozen biopsies coming from different muscles, (2) selective to yield cells uniformly positive for CD56 and negative for CD34 without FACS sorting, (3) reliable in maintaining proliferative and in vitro differentiative capacity up to passage 10.
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Acknowledgements
This work has been funded by Fondazione Istituto di Ricerca Città della Speranza (Grant number 12/01) and CARIPARO-IRP Ricerca Pediatrica Program (Grant number 13/04). Luca Urbani and Paolo De Coppi are supported by GOSH and NIHR (RP-2014-04-046).
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Franzin, C. et al. (2016). Isolation and Expansion of Muscle Precursor Cells from Human Skeletal Muscle Biopsies. In: Turksen, K. (eds) Stem Cell Heterogeneity. Methods in Molecular Biology, vol 1516. Humana Press, New York, NY. https://doi.org/10.1007/7651_2016_321
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DOI: https://doi.org/10.1007/7651_2016_321
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-6549-6
Online ISBN: 978-1-4939-6550-2
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