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Analysis of microRNA Microarrays in Cardiogenesis

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Microarray Data Analysis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1375))

Abstract

microRNAs are a subclass of noncoding RNAs which have been demonstrated to play pivotal roles in multiple cellular mechanisms. microRNAs are small RNA molecules of 22–24 nt in length capable of modulating protein translation and/or RNA stability by base-priming with complementary sequences of the mRNAs, normally at the 3′untranslated region. To date, over 2,000 microRNAs have been already identified in humans, and orthologous microRNAs have been also identified in distinct animals and plants ranging a wide vast of species. High-throughput analyses by microarrays have become a gold standard to analyze the changes on microRNA expression in normal and pathological cellular or tissue conditions. In this chapter, we provide insights into the usage of this uprising technology in the context of cardiac development and disease.

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Author information

Authors and Affiliations

  1. Cardiovascular Development Group, Department of Experimental Biology, University of Jaén, Edificio B-3, Campus Las Lagunillas, Jaen, 23071, Spain

    Diego Franco, Fernando Bonet, Francisco Hernandez-Torres, Estefania Lozano-Velasco & Amelia E. Aranega

  2. System Biology Group, Department of Experimental Biology, University of Jaén, Jaén, Spain

    Francisco J. Esteban

Authors
  1. Diego Franco
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  2. Fernando Bonet
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  3. Francisco Hernandez-Torres
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  4. Estefania Lozano-Velasco
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  5. Francisco J. Esteban
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  6. Amelia E. Aranega
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Corresponding author

Correspondence to Diego Franco .

Editor information

Editors and Affiliations

  1. Department of Surgical and Medical Sciences, University “Magna Græcia” of Catanzaro, Catanzaro, Italy

    Pietro Hiram Guzzi

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Franco, D., Bonet, F., Hernandez-Torres, F., Lozano-Velasco, E., Esteban, F.J., Aranega, A.E. (2015). Analysis of microRNA Microarrays in Cardiogenesis. In: Guzzi, P. (eds) Microarray Data Analysis. Methods in Molecular Biology, vol 1375. Humana Press, New York, NY. https://doi.org/10.1007/7651_2015_247

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  • DOI: https://doi.org/10.1007/7651_2015_247

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3172-9

  • Online ISBN: 978-1-4939-3173-6

  • eBook Packages: Springer Protocols

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