Abstract
Adipose-derived stem cells (ASCs) are multipotent cells that are emerging as an extremely promising therapeutic agent for tissue regeneration. The ability to manipulate ASC phenotypes by the delivery of biologically active cargoes is essential to understand their role and to design novel therapeutic strategies based on the use of ASCs. Here we describe a simple and efficient protocol for the conjugation and efficient delivery of biological materials into ASCs based on the use of polystyrene nanoparticles as carrier system.
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Acknowledgements
This work was supported in part by grants from the Instituto de Salud Carlos III (Fondo de Investigación Sanitaria, FEDER, grant number PI10/02295), the Consejería de Economía, Innovación y Ciencia (Junta de Andalucía, excellence project number CTS-6568), and the Research Executive Agency (REA) of the European Commission (FP7-PEOPLE-2011-CIG-Project Number 294142).
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Sanchez-Martin, R., Cano-Cortés, V., Marchal, J.A., Perán, M. (2013). In Vitro Nanoparticle-Mediated Intracellular Delivery into Human Adipose-Derived Stem Cells. In: Turksen, K. (eds) Stem Cell Nanotechnology. Methods in Molecular Biology, vol 1058. Humana Press, Totowa, NJ. https://doi.org/10.1007/7651_2013_7
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DOI: https://doi.org/10.1007/7651_2013_7
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-570-5
Online ISBN: 978-1-62703-571-2
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