Abstract
Interaction of recombination protein RecO with single-stranded (ss) DNA-binding protein (SSB) is essential for DNA damage repair and restart of stalled replication (Cox, Crit Rev Biochem Mol Biol 42(1):41–63, 2007). To understand mechanism of this interaction and its role in DNA repair, we deciphered a high-resolution structure of RecO complex with C-terminal tail of SSB (SSB-Ct). The structure revealed a key role of hydrophobic interactions between two proteins and suggests the mechanism of RecO recruitment to DNA during homologous recombination and strand annealing.
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Acknowledgement
This work was supported by NIH grant GM073837. The coordinates of RecO–SSB-Ct complex and structure factors were deposited to PBD with ID 3Q8D. We are thankful to Olga Koroleva, who performed all cloning and participated in optimization of protein purification and crystallization procedures, to the staff of GM/CA beamline at APS, and, particularly, to Dr. R. Sanishvili for help in optimization of data collection from small crystals.
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Ryzhikov, M., Korolev, S. (2012). Structural Studies of SSB Interaction with RecO. In: Keck, J. (eds) Single-Stranded DNA Binding Proteins. Methods in Molecular Biology, vol 922. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-032-8_7
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DOI: https://doi.org/10.1007/978-1-62703-032-8_7
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Publisher Name: Humana Press, Totowa, NJ
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