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M13 and Phagemid-Based Cloning Vectors

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Molecular Biomethods Handbook

Part of the book series: Springer Protocols Handbooks ((SPH))

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Abstract

Bacteriophage-based vectors, such as λ have been useful in creating gene llbrarles and in the expression of proteins However, a number of techniques used in molecular biology require DNA to be ina single-stranded form, such as chain termmatlon sequencing, ollgonucleotlde-directed mutagenesis, and certain probe synthesis strategies A small number of naturally occurring viruses produce single-stranded DNA as part of their life cycle, these include M13, fl , and fd, and a number of them have been mampulated and adapted as cloning vectors for the purpose of single-strand DNA production

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Author information

Authors and Affiliations

  1. Unzverszty of Hertfordshzre, Hatfield, UK

    Ralph Rapley

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  1. Ralph Rapley
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Editor information

Editors and Affiliations

  1. University of Herfordshire, Hatfield, UK

    Ralph Rapley  & John M. Walker  & 

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© 1998 Humana Press Inc , Totowa, NJ

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Rapley, R. (1998). M13 and Phagemid-Based Cloning Vectors. In: Rapley, R., Walker, J.M. (eds) Molecular Biomethods Handbook. Springer Protocols Handbooks. Humana Press. https://doi.org/10.1007/978-1-59259-642-3_17

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  • DOI: https://doi.org/10.1007/978-1-59259-642-3_17

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-501-0

  • Online ISBN: 978-1-59259-642-3

  • eBook Packages: Springer Book Archive

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