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RNA Structure Determination

Volume 1490 of the series Methods in Molecular Biology pp 199-215

Date:

Automated RNA 3D Structure Prediction with RNAComposer

  • Marcin BiesiadaAffiliated withEuropean Center for Bioinformatics and Genomics, Institute of Computing Science, Poznan University of TechnologyDepartment of Structural Chemistry and Biology of Nucleic Acids, Institute of Bioorganic Chemistry, Polish Academy of Sciences 
  • , Katarzyna J. PurzyckaAffiliated withDepartment of Structural Chemistry and Biology of Nucleic Acids, Institute of Bioorganic Chemistry, Polish Academy of Sciences 
  • , Marta SzachniukAffiliated withEuropean Center for Bioinformatics and Genomics, Institute of Computing Science, Poznan University of TechnologyDepartment of Bioinformatics, Institute of Bioorganic Chemistry, Polish Academy of Sciences
  • , Jacek BlazewiczAffiliated withEuropean Center for Bioinformatics and Genomics, Institute of Computing Science, Poznan University of TechnologyDepartment of Bioinformatics, Institute of Bioorganic Chemistry, Polish Academy of Sciences
  • , Ryszard W. AdamiakAffiliated withEuropean Center for Bioinformatics and Genomics, Institute of Computing Science, Poznan University of TechnologyDepartment of Structural Chemistry and Biology of Nucleic Acids, Institute of Bioorganic Chemistry, Polish Academy of Sciences Email author 

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Abstract

RNAs adopt specific structures to perform their activities and these are critical to virtually all RNA-mediated processes. Because of difficulties in experimentally assessing structures of large RNAs using NMR, X-ray crystallography, or cryo-microscopy, there is currently great demand for new high-resolution 3D structure prediction methods. Recently we reported on RNAComposer, a knowledge-based method for the fully automated RNA 3D structure prediction from a user-defined secondary structure. RNAComposer method is especially suited for structural biology users. Since our initial report in 2012, both servers, freely available at http://​rnacomposer.​ibch.​poznan.​pl and http://​rnacomposer.​cs.​put.​poznan.​pl have been often visited. Therefore this chapter provides guidance for using RNAComposer and discusses points that should be considered when predicting 3D RNA structure. An application example presents current scope and limitations of RNAComposer.

Key words

RNA tertiary structure RNA three-dimensional structure RNA modeling