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High-Speed Super-Resolution Imaging of Live Fission Yeast Cells

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Yeast Cytokinesis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1369))

Abstract

We describe a step-by-step method for high-speed fluorescence photoactivation localization microscopy (FPALM) of live fission yeast cells. The resolution with this method is tenfold better than spinning disk confocal microscopy.

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Acknowledgements

This work was supported by the National Institute of General Medical Sciences of the National Institutes of Health under award number R01GM026132 to TDP, a grant 095927/A/11/Z from the Wellcome Trust to JB, a James Hudson Brown—Alexander Brown Coxe Postdoctoral Fellowship to FH and a HFSP Long-Term Fellowship to CL. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. JB discloses significant financial interest in Bruker Corp. JB and FH disclose significant financial interest in Hamamatsu Photonics K.K.

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Authors and Affiliations

  1. Department of Molecular Cellular and Developmental Biology, Yale University, 208103, New Haven, CT, 06520-8103, USA

    Caroline Laplante & Thomas D. Pollard

  2. Department of Molecular Biophysics and Biochemistry, Yale University, 208103, New Haven, CT, 06520-8103, USA

    Thomas D. Pollard

  3. Department of Molecular Biophysics and Biochemistry, Yale University, 208103, New Haven, CT, 06520-8103, USA

    Fang Huang, Joerg Bewersdorf & Thomas D. Pollard

  4. Department of Biomedical Engineering, Yale University, 208103, New Haven, CT, 06520-8103, USA

    Joerg Bewersdorf

Authors
  1. Caroline Laplante
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  2. Fang Huang
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  3. Joerg Bewersdorf
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  4. Thomas D. Pollard
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Corresponding author

Correspondence to Thomas D. Pollard .

Editor information

Editors and Affiliations

  1. Departamento de Biología Molecular, Universidad de Cantabria, Facultad de Medicina, Cardenal Herrera Oria s/n, Instituto de Biomedicina y Biotecnología de Cantabria, Universidad de Cantabria, CSIC, Santander, Spain

    Alberto Sanchez-Diaz

  2. Universidad de Salamanca, Instituto de Biología Funcional y Genómica, CSIC, Salamanca, Spain

    Pilar Perez

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Laplante, C., Huang, F., Bewersdorf, J., Pollard, T.D. (2016). High-Speed Super-Resolution Imaging of Live Fission Yeast Cells. In: Sanchez-Diaz, A., Perez, P. (eds) Yeast Cytokinesis. Methods in Molecular Biology, vol 1369. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3145-3_4

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  • DOI: https://doi.org/10.1007/978-1-4939-3145-3_4

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3144-6

  • Online ISBN: 978-1-4939-3145-3

  • eBook Packages: Springer Protocols

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