Abstract
Organisms residing in the oral cavity (oral microbiota) contribute to health and disease, and influence diseases like gingivitis, periodontitis, and oral candidiasis (the most common oral complication of HIV-infection). These organisms are also associated with cancer and other systemic diseases including upper respiratory infections. There is limited knowledge regarding how oral microbes interact together and influence the host immune system. Characterizing the oral microbial community (oral microbiota) in health and disease represents a critical step in gaining insight into various members of this community. While most of the studies characterizing oral microbiota have focused on bacterial community, there are few encouraging studies characterizing the oral mycobiome (the fungal component of the oral microbiota). Our group recently characterized the oral mycobiome in health and disease focusing on HIV. In this chapter we will describe the methods used by our group for characterization of the oral mycobiome.
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Acknowledgement
Funding support is acknowledged from the NIH/NIDCR [RO1DE17846] and the Oral HIV AIDS Research Alliance (BRS-ACURE-S-11-000049-110229 and AI-U01-68636) to MAG; NIH/NIDCR [R01DE024228] to MAG and PKM, NIH/NEI and NIH/NIAID (R21EY021303 and R21AI074077), pilot funding from the Infectious Diseases Drug Development Center (IDDDC, Case), the National Eczema Association (Research Grant), and the National Psoriasis Foundation (Lozick Discovery Research Grant) to PKM, and the CWRU/UH Center for AIDS Research (CFAR, NIH grant number P30 AI036219).
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Chandra, J., Retuerto, M., Mukherjee, P.K., Ghannoum, M. (2016). The Fungal Biome of the Oral Cavity. In: Calderone, R., Cihlar, R. (eds) Candida Species. Methods in Molecular Biology, vol 1356. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3052-4_9
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DOI: https://doi.org/10.1007/978-1-4939-3052-4_9
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-3051-7
Online ISBN: 978-1-4939-3052-4
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