Abstract
Stem cells are cells capable of proliferation, self-renewal, and differentiation into specific phenotypes. They are an essential part of tissue engineering, which is used in regenerative medicine in case of degenerative diseases. In this chapter, we describe the methods of isolating and culturing various types of stem cells, like human embryonic stem cells (hESCs), human umbilical cord derived mesenchymal stem cells (hUC-MSCs), murine bone marrow derived mesenchymal stem cells (mBM-MSCs), murine adipose tissue derived mesenchymal stem cells (mAD-MSCs), and murine bone marrow derived dendritic cells (mBMDCs). All these cell types can be used in tissue engineering techniques.
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Acknowledgements
The authors wish to acknowledge Department of Biotechnology (Government of India) for funding the research work on BJNhem 19 and 20. Further the fellowship for SK was provided by Science and Engineering Research Board (Government of India). The work on H7 was undertaken by ERB in Professor William R. Henderson Jr.’s lab with funding from NIH and under his tutelage. The authors would like to acknowledge collaborators whose intellectual input have been instrumental in mentoring stem cell research in the ERB lab, namely Professors Thalia Papayannopoulou and Charles E. Murry, experts in stem cell biology, the former in hematopoiesis and the latter in cardiomyocyte differentiation.
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Kar, S., Mitra, S., Banerjee, E.R. (2015). Isolation and Culture of Embryonic Stem Cells, Mesenchymal Stem Cells, and Dendritic Cells from Humans and Mice. In: Turksen, K. (eds) Stem Cell Heterogeneity. Methods in Molecular Biology, vol 1516. Humana Press, New York, NY. https://doi.org/10.1007/7651_2015_315
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DOI: https://doi.org/10.1007/7651_2015_315
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-6549-6
Online ISBN: 978-1-4939-6550-2
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