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CEST MRI Reporter Genes

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Magnetic Resonance Neuroimaging

Part of the book series: Methods in Molecular Biology ((MIMB,volume 711))

Abstract

In recent years, several reporter genes have been developed that can serve as a beacon for non-invasive magnetic resonance imaging (MRI). Here, we provide a brief summary of recent advances in MRI reporter gene technology, as well as detailed “hands-on” protocols for cloning, expression, and imaging of reporter genes based on chemical exchange saturation transfer (CEST).

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Acknowledgments

The protocols described in this chapter were developed through funding with NIH Roadmap R21 EB005252 to J.W.M.B.

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Authors and Affiliations

  1. Kennedy Krieger Institute, F.M. Kirby Research Center for Functional Brain Imaging, The Johns Hopkins University School of Medicine, Baltimore, MD, USA

    Guanshu Liu

  2. Russell H. Morgan Department of Radiology and Radiological Science, Division of MR Research, and Cellular Imaging Section, Institute for Cell Engineering, The Johns Hopkins University School of Medicine, Baltimore, MD, USA

    Jeff W.M. Bulte & Assaf A. Gilad

Authors
  1. Guanshu Liu
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  2. Jeff W.M. Bulte
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  3. Assaf A. Gilad
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Corresponding author

Correspondence to Guanshu Liu .

Editor information

Editors and Affiliations

  1. Institute of Psychiatry, Department of Neuroscience, King's College London, Coldharbour Lane 125, London, SE5 9NU, United Kingdom

    Michel Modo

  2. Institute of Cell Engineering, Dept Radiology & Radiological Sciences, Johns Hopkins Univ. Sch. of Med., Rutland Ave. 720, Baltimore, 21205, Maryland, USA

    Jeff W.M. Bulte

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Liu, G., Bulte, J.W., Gilad, A.A. (2011). CEST MRI Reporter Genes. In: Modo, M., Bulte, J. (eds) Magnetic Resonance Neuroimaging. Methods in Molecular Biology, vol 711. Humana Press. https://doi.org/10.1007/978-1-61737-992-5_13

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  • DOI: https://doi.org/10.1007/978-1-61737-992-5_13

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61737-991-8

  • Online ISBN: 978-1-61737-992-5

  • eBook Packages: Springer Protocols

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