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PCR Detection of Haemophilus influenzae from Respiratory Specimens

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PCR Detection of Microbial Pathogens

Part of the book series: Methods in Molecular Biology ((MIMB,volume 943))

Abstract

The detection of Haemophilus influenzae by conventional methods like culture is time-consuming and may give false-negative results, especially during ongoing antibiotic treatment. Therefore, non-culture based methods that are sensitive, specific, and rapid are valuable for early diagnosis and effective therapy. Here we describe a quantitative real-time PCR assay based on the outer membrane P6 gene omp6, to detect H. influenzae and its application on respiratory tract specimens.

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Correspondence to Björn Herrmann .

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Abdeldaim, G.M.K., Herrmann, B. (2013). PCR Detection of Haemophilus influenzae from Respiratory Specimens. In: Wilks, M. (eds) PCR Detection of Microbial Pathogens. Methods in Molecular Biology, vol 943. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60327-353-4_7

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  • DOI: https://doi.org/10.1007/978-1-60327-353-4_7

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-60327-352-7

  • Online ISBN: 978-1-60327-353-4

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