Summary
Anopheles mosquitoes are the major vectors of human malaria parasites. Mosquito–parasite interactions are critical for disease transmission and therefore represent a potential target for malaria control strategies. Mosquitoes mount potent antiparasitic responses, and identification of mosquito factors that limit parasite development is one of the major objectives in the field. To address this question, we have developed a convenient reverse genetics approach by injection of double-stranded RNA (dsRNA) in adult mosquitoes, to evaluate the function of candidate genes in mosquito antiparasitic responses.
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References
Richman, A. M., Dimopoulos, G., Seeley, D., and Kafatos, F. C. (1997) Plasmodium activates the innate immune response of Anopheles gambiae mosquitoes. EMBO J. 16, 6114–6119.
Vizioli, J., Bulet, P., Charlet, M., Lowenberger, C., Blass, C., Muller, H. M., Dimopoulos, G., Hoffmann, J., Kafatos, F. C., and Richman, A. (2000) Cloning and analysis of a cecropin gene from the malaria vector mosquito, Anopheles gambiae. Insect Mol. Biol. 9, 75–84.
Dimopoulos, G., Christophides, G. K., Meister, S., Schultz, J., White, K. P., Barillas-Mury, C., and Kafatos, F. C. (2002) Genome expression analysis of Anopheles gambiae: responses to injury, bacterial challenge, and malaria infection. Proc. Natl. Acad. Sci. USA 99, 8814–8819.
Han, Y. S., and Barillas-Mury, C. (2002) Implications of Time Bomb model of ookinete invasion of midgut cells. Insect Biochem. Mol. Biol. 32, 1311–1316.
Janse, C. J., Franke-Fayard, B., Mair, G. R., Ramesar, J., Thiel, C., Engelmann, S., Matuschewski, K., van Gemert, G. J., Sauerwein, R. W., and Waters, A. P. (2006) High efficiency transfection of Plasmodium berghei facilitates novel selection procedures. Mol. Biochem. Parasitol. 145, 60–70.
Blandin, S., Shiao, S. H., Moita, L. F., Janse, C. J., Waters, A. P., Kafatos, F. C., and Levashina, E. A. (2004) Complement-like protein TEP1 is a determinant of vectorial capacity in the malaria vector Anopheles gambiae. Cell 116, 661–670.
Franke-Fayard, B., Trueman, H., Ramesar, J., Mendoza, J., van der Keur, M., van der Linden, R., Sinden, R. E., Waters, A. P., and Janse, C. J. (2004) A Plasmodium berghei reference line that constitutively expresses GFP at a high level throughout the complete life cycle. Mol. Biochem. Parasitol. 137, 23–33.
Blandin, S., Moita, L. F., Kocher, T., Wilm, M., Kafatos, F. C., and Levashina, E. A. (2002) Reverse genetics in the mosquito Anopheles gambiae: targeted disruption of the Defensin gene. EMBO Rep. 3, 852–856.
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Blandin, S.A., Levashina, E.A. (2008). Reverse Genetics Analysis of Antiparasitic Responses in the Malaria Vector, Anopheles gambiae . In: Ewbank, J., Vivier, E. (eds) Innate Immunity. Methods in Molecular Biology™, vol 415. Humana Press. https://doi.org/10.1007/978-1-59745-570-1_21
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DOI: https://doi.org/10.1007/978-1-59745-570-1_21
Publisher Name: Humana Press
Print ISBN: 978-1-58829-746-4
Online ISBN: 978-1-59745-570-1
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