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Detection of GFP During Nervous System Development in Drosophila melanogaster

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Reporter Genes

Part of the book series: Methods in Molecular Biology ((MIMB,volume 411))

Abstract

Using the vital marker GFP and its spectral variants, it is possible to visualize multiple proteins in individual cells and thereby monitor embryonic development on a cellular and molecular level. In the following chapter we describe how to prepare Drosophila embryos or larvae for live imaging or immunohistochemical staining and provide some guidelines for optimal GFP detection.

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Author information

Authors and Affiliations

  1. Wellcome Trust/Cancer Research UK Gurdon Institute, UK

    Karin Edoff, James S. Dods & Andrea H. Brand

  2. Department of Physiology, Development and Neuroscience, University of Cambridge, UK

    Karin Edoff, James S. Dods & Andrea H. Brand

Authors
  1. Karin Edoff
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  2. James S. Dods
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  3. Andrea H. Brand
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Editor information

Editors and Affiliations

  1. Department of Genetic Medicine, Women’s and Children’s Hospital, Adelaide, Australia

    Donald S. Anson

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© 2007 Humana Press Inc., Totowa, NJ

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Edoff, K., Dods, J.S., Brand, A.H. (2007). Detection of GFP During Nervous System Development in Drosophila melanogaster . In: Anson, D.S. (eds) Reporter Genes. Methods in Molecular Biology, vol 411. Humana Press. https://doi.org/10.1007/978-1-59745-549-7_6

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  • DOI: https://doi.org/10.1007/978-1-59745-549-7_6

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-739-6

  • Online ISBN: 978-1-59745-549-7

  • eBook Packages: Springer Protocols

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