Abstract
The magnitude and breadth of T-cell responses against HCV are associated with the outcome of HCV infection. Parameters of HCV-specific T-cell responses that are frequently assessed in clinical immunological studies include proliferation of T cells in response to HCV antigens, frequency of HCV-specific T cells secreting cytokines, and changes in antigen specificity during the course of HCV infection. Common techniques for assessing these parameters such as 3H-thymidine incorporation assay, cytokine ELISpot assay, and strategies for epitope mapping and identification of minimal optimal epitopes are outlined, and detailed protocols are described.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Hoofnagle, J. H. (2002) Course and outcome of hepatitis C. Hepatology 36, S21–S29.
Rehermann, B. and Nascimbeni, M. (2005) Immunology of hepatitis B virus and hepatitis C virus infection. Nat. Rev. Immunol. 5, 215–229.
Chisari, F. V. (2005) Unscrambling hepatitis C virus-host interactions. Nature 436, 930–932.
Shoukry, N. H., Cawthon, A. G., and Walker, C. M. (2004) Cell-mediated immunity and the outcome of hepatitis C virus infection. Annu. Rev. Microbiol. 58, 391–424.
Diepolder, H. M., Zachoval, R., Hoffmann, R. M., Wierenga, E. A., Santantonio, T., Jung, M. C., et al. (1995) Possible mechanism involving T lymphocyte response to non-structural protein 3 in viral clearance in acute hepatitis C virus infection. Lancet 346, 1006–1007.
Thimme, R., Oldach, D., Chang, K. M., Steiger, C., Ray, S. C., and Chisari, F. V., (2001) Determinants of viral clearance and persistence during acute hepatitis C virus infection. J. Exp. Med. 194, 1395–1406.
Thimme, R., Bukh, J., Spangenberg, H. C., Wieland, S., Pemberton, J., Steiger, C., et al. (2002) Viral and immunological determinants of hepatitis C virus clearance, persistence and disease. Proc. Natl. Acad. Sci. USA 99, 15661–15668.
Wedemeyer, H., He, X. S., Nascimbeni, M., Davis, A. R., Greenberg, H. B., Hoofnagle, J. H., et al. (2002) Impaired effector function of hepatitis C virus-specific CD8+ T cells in chronic hepatitis C virus infection. J. Immunol. 169, 3447–3458.
Rehermann, B. and Naoumov, N. V. (2007) Immunological techniques in viral hepatitis. J. Hepatol. 46, 508–520.
Lauer, G. M., Barnes, E., Lucas, M., Tim, J., Ouchi, K., Kim, A. Y., et al. (2004) High resolution analysis of cellular immune responses in resolved and persistent hepatitis C virus infection. Gastroenterology 127, 924–936.
Nascimbeni, M. and Rehermann, B. (2004) Determination of hepatitis B virus-specific CD8+ T-cell activity in the liver. Methods Mol. Med. 96, 65–83.
Acknowledgments
This study was supported by the Intramural Research Program of the National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2009 Humana Press, a part of Springer Science+Business Media, LLC
About this protocol
Cite this protocol
Yoon, J.C., Rehermann, B. (2009). Determination of HCV-Specific T-Cell Activity. In: Tang, H. (eds) Hepatitis C. Methods in Molecular Biology™, vol 510. Humana Press. https://doi.org/10.1007/978-1-59745-394-3_30
Download citation
DOI: https://doi.org/10.1007/978-1-59745-394-3_30
Publisher Name: Humana Press
Print ISBN: 978-1-58829-970-3
Online ISBN: 978-1-59745-394-3
eBook Packages: Springer Protocols