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Anthocyanins in cowpea [Vigna unguiculata (L.) Walp. ssp. unguiculata]

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Abstract

The aim of this study was to isolate and identify the anthocyanins in the black seed coated cowpea [Vigna unguiculata (L.) Walp. ssp. unguiculata] using reverse phase C-18 open column chromatography and high performance liquid chromatography (HPLC) with diode array detection and electro spray ionization/mass spectrometry (DAD-ESI/MS) analysis, respectively. Anthocyanins were extracted from the coat of black cowpea with 1% trifluoroacetic acid (TFA) in methanol, isolated by RP-C-18 column chromatography, and their structures elucidated by 1D and 2D nuclear magnetic resonance (NMR) spectroscopy. The isolated anthocyanins were characterized as delphinidin-3-O-glucoside (2) and cyanidin-3-O-glucoside (4). Furthermore, 5 minor anthocyanins were detected and identified as delphinidin-3-O-galactoside (1), cyanidin-3-O-galactoside (3), petunidin-3-O-glucoside (5), peonidin-3-O-glucoside (7), and malvidin-3-O-glucoside (8) based on the fragmentation patterns of HPLC-DADESI/MS analysis.

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Authors and Affiliations

  1. Department of Functional Crop, National Institute of Crop Science (NICS), Rural Development Administration, Miryang, Gyeongnam, 627-803, Korea

    Tae Joung Ha, Myoung-Hee Lee, Yu Na Jeong, Jin Hwan Lee, Sang-Ik Han, Chang-Hwan Park, Suk-Bok Pae, Chung-Dong Hwang, In-Youl Baek & Keum-Yong Park

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  1. Tae Joung Ha
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  2. Myoung-Hee Lee
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  3. Yu Na Jeong
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  4. Jin Hwan Lee
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  5. Sang-Ik Han
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  7. Suk-Bok Pae
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  8. Chung-Dong Hwang
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  9. In-Youl Baek
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  10. Keum-Yong Park
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Correspondence to Tae Joung Ha.

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Ha, T.J., Lee, MH., Jeong, Y.N. et al. Anthocyanins in cowpea [Vigna unguiculata (L.) Walp. ssp. unguiculata]. Food Sci Biotechnol 19, 821–826 (2010). https://doi.org/10.1007/s10068-010-0115-x

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  • DOI: https://doi.org/10.1007/s10068-010-0115-x

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