Abstract
Organophosphate compounds are nowadays the most frequently used pesticides. For these insecticides, the primary target is acetylcholinesterase and for this reason the main clinical effect of acute intoxication with organophosphate insecticides involves an irreversible inhibition of the activity of this enzyme. However, in the chronic or subchronic exposition oxidative stress has been reported as the main mechanism of its toxicity. The present study investigated the effect of three low doses (0.2, 2, 5 mg/kg bw) of chlorpyrifos for 14 or 28 days on serum liver enzymes and on oxidative stress parameters in the liver of rats. Chlorpyrifos treatment resulted in aminotransferases and alkaline phosphatase increase after 14 days (higher doses) and 28 days (all doses) treatment together with changes of antioxidative enzymes activities and reduced glutathione and malonyldialdehyde level in the liver. The enhancement of lipid peroxidation is temporary, reaching a peak after 14 days and decreasing after 28 days of treatment. Based on the experimental findings of this study the temporary liver injury caused by oxidative stress has been shown. The disturbances in the liver antioxidative status and increased liver membrane permeability may appear in case of doses near to the accepted human daily intake.
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References
Lukaszewicz-Hussain A. Role of oxidative stress in organophosphates insecticide toxicity - short review, Pest. Biochem. Physiol., 2010, 98, 45–150
Rezg R., Mornagui B., El-Fazaa S., Garbi N. Organophosphorus pesticides as food chain contaminants and type 2 diabetes: a review, Trends Food Sci. Technol., 2010, 21, 345–357
Curl C.L., Fenske R.A., Kissel J.C., Shirai J.H., Moate T.F., Griffith W., Coronado G., Thompson B. Evaluation of take-home organophosphorus pesticide exposure among agricultural workers and their children, Environ. Health Perspect., 2002, 110, A787–792
Lotti M. Clinical toxicology of anticholinesterase agents in humans. In: Krieger R.I., Doull J., editors. Handbook of pesticide toxicology, Volume 2. Agents. San Diego, USA, Academic Press, USA, 2001, 1043–1085
Savolainen K. Understanding the toxic action of organophosphates. In: Krieger R.I., Doull J., editors. Handbook of pesticide toxicology, Volume 2. Agents. San Diego, USA, Academic Press, USA, 2001, Handbook of Pesticide Toxicology, II Ed, Academic Press, USA, 2001, 1013–1043
Yurumez Y., Cemek M., Yavuz Y., Birdane Y.O. Beneficial effect of N-acetylcysteine against organophosphate toxicity in mice, Biol. Pharmacol. Bull., 2007, 30, 490–494
Possamai F.P., Fortunato J.J., Feier G., Agostinho F.R., Quevedo J., Filho D.W., Dal-Pizzol F. Oxidative stress after acute and sub-chronic malathion intoxication in Wistar rats, Environ. Toxicol. Pharmacol., 2007, 23, 198–204
Lukaszewicz-Hussain A., Moniuszko-Jakoniuk J. Activities of superoxide dismutase and catalase in erythrocytes and concentration of malondialdehyde in serum of rats intoxicated with chlorfenvinphos in low doses, Pol. J. Environ. Stud., 1999, 8, 234–236
Lukaszewicz-Hussain A., Moniuszko-Jakoniuk J., Rogalska J. Assessment of lipid peroxidation in rat tissues in subacute chlorfenvinphos administration, Pol. J. Environ. Stud. 2007, 16, 233–236
Lukaszewicz-Hussain A. Subchronic intoxication with chlorfenvinphos, an organophosphate insecticide, affects rat brain antioxidative enzymes and glutathione level, Food Chem. Toxicol., 2008, 46, 82–86
Mates J.M., Perez-Gomez C., Nunez De Castro I. Antioxidant enzymes and human diseases, Clin. Biochem., 1999, 32, 595–603
Morgan M.J., Kim Y.S., Liu Z. Lipids rafts and oxidative stress-induced cell death, Antiox. Redox Sign., 2007, 9, 1–13
Gerard-Monnier D., Chaudiere J. Metabolism and antioxidant function of glutathione, Pathol. Biol. (Paris), 1996, 44, E209–E214
Spolarics Z., Wu J.X. Role of glutathione and catalase in H2O2 detoxification in LPS-activated hepatic endothelial and Kupffer cells, Am. J. Physiol., 1997, 273, G1304–G1311
Lukaszewicz-Hussain A. Activities of brain antioxidant enzymes, lipid and protein peroxidation, Central Eur. J. Med., 2011, 6, 588–594
Ajiboye T.O. Redox status of the liver and kidney of 2,2-dichlorovinyl dimethyl phosphate (DDVP) treated rats, Chem. Biol. Interact., 2010, 185, 202–207
Rej R. Aspartate aminotransferase activity and isoenzyme proportions in human liver tissues, Clin. Chem., 1978, 24, 1971–1979
Sturgill M.G., Lambert G.H. Xenobiotic-induced hepatotoxicity: mechanisms of liver injury and methods of monitoring hepatic function, Clin. Chem., 1997, 43, 1512–1526
Yaman H., Isbilir S., Cakir E., Uysal B. Current issues with paracetamol induced toxicity, J. Exp. Integrat. Med., 2011, 1, 165–166
Toxicological profile for chlorpyrifos, U.S. Department of Health and Human Services, Public Health Service, Agency for Toxic Substances and Disease Registry, 1997
Tomlin C.D.S., The Pesticide Manual, A World Compendium, 14th ed.; British Crop Protection Council: Alton, Hampshire, UK, 2006, 186–187
Sahin E., Gümüşlü S. Immobilization stress in rat tissues: alteractions in protein oxidation, lipid peroxidation and antioxidant defense system, Comp. Biochem. Physiol. C. Toxicol. Pharmacol., 2007, 144, 342–347
Aebi H.E. Catalase in vitro, Methods Enzymol., 1984, 105, 121–126
Lowry O.H., Rosenbrough M.J., Farr A.L., Randall R. Protein measurement with the Folin phenol reagent, J. Biol. Chem., 1951, 193, 265–275
FAO Specifications and evaluations for agricultural pesticides. Chlorpyrifos, Food and agriculture organization of the United Nations, 2006
Sharma Y., Bashir S., Irshad M., Gupta S.D., Dogra T.D. Effects of acute dimethoate administration on antioxidant status of liver and brain of experimental rats, Toxicology, 2005, 206, 49–57
Fortunato J.J., Agostinho F.R., Reus G.Z., Petronilho F.C., Dal-Pizzol F., Quevedo J. Lipid peroxidative damage on malathion exposure in rats, Neurotox. Res., 2006, 9, 23–28
Ranjbar A., Solhi H., Mashayekhi F.J., Susanabdi A., Rezaiec A., Abdollahi M. Oxidative stress in acute human poisoning with organophosphorus insecticides; a case control study, Environ. Toxicol. Pharmacol., 2005, 20, 88–91
Lee T.H., Kim W.R., Benson J.T., Therneau T.M., Melton L.J. Serum aminotransferase activity and mortality risk in a United States community, Hepatology, 2008, 47, 880–887
Reichling J.J., Kaplan M.M. Clinical use of serum enzymes in liver disease, Dig. Dis. Sci., 1988, 33, 1601–1614
Akhgari M., Abdollahi M., Kebryaeezadeh A., Hosseini R., Sabzevari O. Biochemical evidence for free radical-induced lipid peroxidation as a mechanism for subchronic toxicity of malathion in blood and liver of rats, Hum. Exp. Toxicol., 2003, 22, 205–208
Rao J.V. Toxic effects of novel organophosphorus insecticide (RPR-V) on certain biochemical parameters of euryhaline fish, Oreochromis mossambicus, Pest. Biochem. Physiol., 2006, 86, 78–84
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Lukaszewicz-Hussain, A. Involvement of oxidative stress in liver injury after subchronic intoxication with low doses of chlorpyrifos — study on rats. cent.eur.j.med 8, 132–139 (2013). https://doi.org/10.2478/s11536-012-0100-2
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DOI: https://doi.org/10.2478/s11536-012-0100-2