Abstract
The diversity and complexity of Bioinformatics tools currently available for protein sequence analysis can make it difficult to know where to begin when presented with a new sequence. In this chapter we assume that the reader has a protein sequence (full-length or partial) identified from mass spectrometry or translation of a putative gene and wishes to identify aspects of its structure and function via Bioinformatics. We go through a protocol outlining one approach that should give the most complete picture possible given the limits of available tools, and then provide a worked example to illustrate the procedures involved. The nature of this paper is such that we are unable to give complete details of all the methods discussed. We refer the reader to references and websites described in the text for more information.
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Copley, R.R., Russell, R.B. (2003). Getting the Most from Your Protein Sequence. In: Smith, B.J. (eds) Protein Sequencing Protocols. Methods in Molecular Biology™, vol 211. Humana Press. https://doi.org/10.1385/1-59259-342-9:411
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DOI: https://doi.org/10.1385/1-59259-342-9:411
Publisher Name: Humana Press
Print ISBN: 978-0-89603-975-9
Online ISBN: 978-1-59259-342-2
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