Abstract
This study was designed to analyze the acute effects of unilateral or bilateral ovariectomy or adrenalectomy, performed on different days of the estrous cycle, on progesterone (P4) serum levels 1 h after surgery. The effects of blocking the cholinergic system by injecting atropine sulfate were also analyzed. Ether anesthesia treatment on diestrus 1 (D1) increased P4 serum levels. Compared to right sham-operated animals, right ovariectomy (left ovary in situ) performed on diestrus 2 (D2) or proestrus (P), resulted in P4 serum levels increase. Compared to animals with left sham surgery, left adrenalectomy (right adrenal in situ) performed on P day resulted in significantly lower P4 concentrations. Bilateral adrenalectomy resulted in a significant drop of P4 serum levels; the most remarkable drop was observed in animals treated on D2. Bilateral ovariectomy performed on D1 resulted in lower P4 serum levels, and the same treatment performed on P resulted in a significant rise of P4 serum levels. Injecting atropine sulfate to untouched (control group) rats resulted in significantly higher P4 concentrations. Blocking the cholinergic system on D1 or P to rats with the right adrenal removed resulted in lower P4 serum levels; while, in contrast, atropine sulfate treatment performed on D2 resulted in P4 serum levels increase. The results support the hypothesis of asymmetry in the ovaries’ and adrenals’ capacities to secrete P4; that this capacity varies along the estrous cycle; and that P4 secretion by the ovaries and adrenals is regulated by the cholinergic system.
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Kalantaridou, S. N., Makrigiannakis, A., Zoumakis, E., and Chrousos, G. P. (2004). J. Reprod. Immunol. 62, 61–68.
Smith, M. S., Freeman, M. E., and Neill, J. D. (1975). Endocrinology 96, 219–226.
De Geyter, C., De Geyter, M., Huber, P. R., Nieschlag, E., and Holzgreve, W. (2002). Hum. Reprod. 17, 933–939.
Matzuk, M. M., Finegold, M. J., Mather, J. P., Krummen, L., Lu, H., and Bradley, A. (1994). Proc. Natl. Acad. Sci. USA 91, 8817–8821.
Wu, X. K., Zhou, S. Y., Sallinen, K., Pollanen, P., and Erkkola, R. (2000). Eur. J. Endocrinol. 143, 383–388.
Domínguez, R., Morales, L., and Cruz, M. E. (2003). Ann. Rev. Biol. Sci. 5, 95–104.
Gerendai, I., Tóth, I. E., Boldogkoi, Z., Medveczky, I., and Halász, B. (2000). J. Auton. Nervous Syst. 80, 40–45.
Frankel, A. I., Chapman, J. C., and Cook, B. (1989). J. Endocrinol. 122, 485–488.
Barco, A. I., Flores, A., Chavira, R., Damian-Matzumura, P., Domínguez, R., and Cruz, M. E. (2003). Endocrine 21, 209–215.
Domínguez, R., Riboni, L., Zipitría, D., and Revilla, R. (1982). J. Endocrinol. 95, 175–180.
Domínguez, R., Cruz, M. E., and Chávez, R. (1988). In: Growth factors and the ovary. Hirshfield, A. N. (eds.). Plenum Press: New York, 39, 321–325.
Bateman, A., Singh, A., Oral, Kral, T., and Solomon, S. (1989). Endocrine Rev. 10, 92–111.
Shors, T. J., Pickett, J., Wood, G., and Paczynski, M. (1999). Stress 3, 162–171.
Bailey, K. J. (1987). J. Endocrinol. 112, 15–21.
Brown, G. P., Courtney, G. A., and Marotta, S. F. (1976). Steroids 28, 283–294.
Judd, S., Terry, A., Petrucco, M., and White, G. (1992). J. Clin. Endocrinol. Metab. 74, 299–305.
Lobo, R. A., Goebelsmann, U., Brenner, P. F., and Mishell, D. R. Jr. (1982). Am. J. Obstet. Gynecol. 142, 471–478.
Davoren, B. J. and Hsueh, A. J. W. (1984). Excerpta Medica 7th Int. Congr. Endocrinol. 652, 490.
Ahmed, C. E., Dees, W. L., and Ojeda, S. R. (1986). Endocrinology 118, 1682–1689.
Ojeda, S. R., Costa, M. E., Katz, K. H., and Hersh, L. B. (1985). Biol. Reprod. 33, 286–295.
Adashi, E. Y. and Hsueh, A. J. (1981). Endocrinology 108, 2170–2178.
Aguado, L. I., Petrovic, S. L., and Ojeda, S. R. (1982). Endocrinology 110, 1124–1132.
Ferruz, J., Barria, A., Galleguillos, S., and Lara, H. E. (1991). Biol. Reprod. 45, 592–597.
Morales, L., Chávez, R., Ayala, M. E., and Domínguez, R. (1998). J. Endocrinol. 158, 213–219.
Morán, C., Morales, L., Quiroz, U., and Domínguez, R. (2000). J. Endocrinol. 166, 205–211.
Aguado, L. I. (2002). Microsc. Res. Tech. 59, 462–473.
De Bortoli, M. A., Garraza, M. H., and Aguado, L. I. (1998). J. Endocrinol. 159, 61–68.
De Bortoli, M. A., Garraza, M. H., and Aguado, L. I. (2002). Neuroendocrinol. Lett. 23, 27–31.
Klein, C. M., Ray, R. H., and Burden, H. W. (1989). Brain Res. 479, 194–200.
Galvez, A., Paredes, A., Fiedler, J. L., Venegas, M., and Lara, H. E. (1999). Endocrine 10, 131–135.
Aguado, L. I. and Ojeda, S. R. (1984). Endocrinology 114, 1845–1853.
Morán, C., Franco, A., Morán, J. L., Handal, A., Morales, L., and Domínguez, R. (2005). Endocrine 26, 147–152.
Saper, C. B., Loewy, A. D., Swanson, L. W., and Cowan, W. M. (1976). Brain Res. 117, 305–316.
Baljet, B. and Drukker, J. (1980). Acta Anat. 107, 241–267.
Klein, C. M. and Burden, H. W. (1988). Neurosci. Lett. 85, 217–222.
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Flores, A., Meléndez, G., Palafox, M.T. et al. The participation of the cholinergic system in regulating progesterone secretion through the ovarian-adrenal crosstalk varies along the estrous cycle. Endocr 28, 145–151 (2005). https://doi.org/10.1385/ENDO:28:2:145
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DOI: https://doi.org/10.1385/ENDO:28:2:145