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A novel composite scaffold for cardiac tissue engineering

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Summary

One approach to the engineering of functional cardiac tissue for basic studies and potential clinical use involves bioreactor cultivation of dissociated cells on a biomaterial scaffold. Our objective was to develop a scaffold that is (1) highly porous with large intereconnected pores (to facilitate mass transport), (2) hydrophilic (to enhance cell attachment), (3) structurally stable (to withstand the shearing forces during bioreactor cultivation), (4) degradable (to provide ultimate biocompatibility of the tissue graft), and (5) elastic (to enable transmission of contractile forces). The scaffold of choice was made as a composite of poly(Dl-lactide-co-caprolactone), poly(Dl-lactide-co-glycolide) (PLGA), and type I collagen, with open interconnected pores and the average void volume of 80±5%. Neonatal rat heart cells suspended in Matrigel were seeded into the scaffold at a physiologically high density (1.35×108 cells/cm3) and cultivated for 8 d in cartridges perfused with culture medium or in orbitally mixed dishes (25 rpm); collagen sponge (Ultrafoam⋆m) and PLGA sponge served as controls. Construct cellularity, presence of cardiac markers, and contractile properties were markedly improved in composite scaffolds as compared with both controls.

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Authors and Affiliations

  1. Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, 02139, Cambridge, Massachusetts

    Hyoungshin Park, Milica Radisic & Gordana Vunjak-Novakovic

  2. Medical Research Institute, Kyungpook National University, 700-721, Daegu, Korea

    Jeong Ok Lim

  3. Institute for Regenerative Medicine, Wake Forest School of Medicine, 27157, Winston Salem, North Carolina

    Jeong Ok Lim

  4. Department of Thoracic and cardiovascular Surgery, Kyungpook National University, School of Medicine, 700-721, Daegu, Korea

    Bong Hyun Chang

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  1. Hyoungshin Park
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  2. Milica Radisic
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  3. Jeong Ok Lim
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  4. Bong Hyun Chang
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  5. Gordana Vunjak-Novakovic
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Correspondence to Gordana Vunjak-Novakovic.

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Park, H., Radisic, M., Lim, J.O. et al. A novel composite scaffold for cardiac tissue engineering. In Vitro Cell.Dev.Biol.-Animal 41, 188–196 (2005). https://doi.org/10.1290/0411071.1

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  • DOI: https://doi.org/10.1290/0411071.1

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