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Characterizing the structure of protein layers adsorbed onto functionalized surfaces by means of in-situ X-ray reflectivity

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Abstract

In a biological cell, proteins face a highly complex environment comprising crowding and confinement effects as well as interactions with interfaces, cosolvents, and other biomolecules. In this study, the X-ray reflectivity technique has been used for the in-situ characterization of adsorbed protein layers at solid-liquid interfaces. The adsorption of bovine serum albumin at the hydrophobic polystyrene-water interface has been investigated in the presence and in the absence of salts. The data indicate that enhanced adsorption occurs at high salt concentrations.

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Evers, F., Shokuie, K., Paulus, M. et al. Characterizing the structure of protein layers adsorbed onto functionalized surfaces by means of in-situ X-ray reflectivity. Eur. Phys. J. Spec. Top. 167, 185–189 (2009). https://doi.org/10.1140/epjst/e2009-00956-1

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